顶空固相微萃取法结合气相色谱-质谱联用法分析2种滇产艾纳香的挥发性成分

Analysis of volatile components of 2 kinds of Blumea balsamifera collected from Yunnan by headspace solid phase microextraction-gas chromatography-mass spectrometry

中文关键词:  艾纳香  挥发性成分  顶空固相微萃取  气相色谱-质谱联用法

英文关键词:Blumea balsamifera  volatile components  headspace solid phase microextraction  gas chromatography-mass spectrometry

中文摘要:

      目的 采用顶空固相微萃取法结合气相色谱-质谱联用技术(HS-SPME-GC-MS)对西双版纳和保山2种滇产艾纳香的挥发性成分进行研究, 揭示其主要香气成分并比较产地差异。方法 样品经50 ℃加热1.0 h, 顶空富集30 min, 在250 ℃解析2.0 min后利用GC-MS进行测定, 对其香气化学成分进行分析, 并以面积归一化法测定各个成分的相对含量。结果 从2种艾纳香中分别分离鉴定了57、53种化合物, 占各自挥发性成分总量的98.32%和98.14%, 其中2种艾纳香的共有化合物为52种。樟脑、(-)龙脑、β-石竹烯、α-蒎烯、β-蒎烯、莰烯等6种香气成分占版纳艾纳香挥发性成分总量的86.31%, 占保山艾纳香82.00%。樟脑、(-)龙脑、β-石竹烯等3种成分占版纳艾纳香挥发性成分总量的63.02%, 保山的为48.10%。结论 西双版纳及保山2种滇产艾纳香的挥发性成分种类基本一致, 且具有6种共同的主要香气成分, 但6种主要香气成分的相对含量存在明显不同, 保山艾纳香中的樟脑、(-)龙脑、β-石竹烯3种香气成分含量明显更丰富, 而另外3种成分低于版纳艾纳香。研究结果可为滇产艾纳香的开发利用提供了科学依据。

英文摘要:

      Objective To study the volatile components of 2 kinds of Blumea balsamifera collected from Xishuangbanna (XSBN) and Baoshan (BS) by headspace solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS) and to reveal the composition characteristics of the main aroma components and compare the regional differences of aroma components. Methods Samples were heated at 50 ℃ for 1.0 h, enriched in headspace for 30 min, and analyzed at 250 ℃ for 2.0 min before being used for determination by GC-MS. The chemical components of the aroma were analyzed, and the relative content of each component was determined by area normalization method. Results Totally 57 and 53 compounds including 52 common ingredients were isolated and identified from the 2 experiment samples from XSBN and BS, respectively, accounting for 98.32% and 98.14% of the total volatile components. The most abundant 6 aromatic components, including camphor, (-)borneol, β-carnitene, α-pinene, β-pinene, and camphene accounted for 86.31% of the total volatile components in sample from XSBN, 82.00% of the samples from BS. Camphor, (-)borneol and β-caryophyllene accounted for 63.02% and 48.10% of the total volatile components in XSBN and BS, respectively. Conclusion The volatile components of 2 kinds of Blumea balsamifera from Xishuangbanna and Baoshan are basically the same, and they have 6 common main aroma components, but the relative content of the 6 main aroma components is significantly different. The relative content of camphor, (-)borneol and β-carnitene is much higher in XSBN’s sample, while the content of other 3 components are lower than XSBN’s. The results of this study can provide scientific basis for the development and utilization of Blumea balsamifera producing in Yunnan province.