胶体金免疫检测水产品中孔雀石绿残留的前处理方法优化

Optimization of pretreatment method for the determination of malachite green residue in aquatic products by colloidal gold immunoassay

中文关键词:  孔雀石绿  胶体金免疫层析现场快速检测  样品前处理

英文关键词:malachite green  colloidal gold immunochromatographic rapid detection  sample preparation

中文摘要:

      目的 优化胶体金免疫检测水产品中孔雀石绿残留的前处理方法。方法 采用超静音可调节式气泵代替氮吹仪或空气吹干仪, 对商品化孔雀石绿胶体金免疫层析试剂盒前处理方法中的样品用量、提取剂用量、吹干体积量及吹干温度等条件进行优化, 并将优化后的前处理方法在10种水产品中进行应用验证。结果 在保证原有试剂盒检出限2 μg/kg的前提下, 减少样品用量和提取剂用量, 节约检测成本; 不需用大型仪器, 降低检测人员操作要求; 样品前处理步骤更加简便, 单个样品前处理和检测总时间少于15 min; 在10种水产品中具有较好的实际适用性。结论 优化后的孔雀石绿免疫胶体金层析试剂盒前处理方法更加简便、快捷, 可实现水产品中孔雀石绿的基层现场快速检测。

英文摘要:

      Objective To optimize the pretreatment method for the determination of malachite green residue in aquatic products by colloidal gold immunoassay. Methods Ultra-quiet adjustable air pump was used to replace the nitrogen blowing instrument or air blowing-drying instrument, and the pretreatment conditions of commercial malachite green colloidal gold immunochromatography kit, such as sample dosage, extractant dosage, blowing-drying volume and blowing-drying temperature were optimized. And the optimized pretreatment method was applied to 10 kinds of aquatic products for verification. Results On the premise of ensuring the detection limit of the original kit to be 2 μg/kg, the sample dosage and the extraction agent dosage were reduced, and the detection cost was saved. No large-scale instruments were needed to reduce the operation requirements of testing personnel. The sample pretreatment steps were simpler, and the total pretreatment and detection time of a single sample was less than 15 min. It had good practical applicability in 10 kinds of aquatic products. Conclusion The optimized pretreatment method of the malachite green colloidal gold immunochromatography kit is simpler and faster, which can be used for rapid detection of malachite green grassroots places in the aquatic products.