超高效液相色谱-串联质谱法同时测定保健酒中14种黄酮类成分的含量

Simultaneous determination of 14 kinds of flavones in health-care wine by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  保健酒  黄酮类  超高效液相色谱-串联质谱法

英文关键词:health-care wine  flavones  ultra performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立超高效液相色谱-串联质谱法同时测定保健酒中14种黄酮类成分含量的方法。方法 样品经乙腈水(2:8, V:V)稀释10倍后, 采用Luna?C8(2) 100A (150 mm× 2 mm, 3 μm)柱以0.1%甲酸溶液和乙腈为流动相进行梯度洗脱分离, 电喷雾负离子化(electron spray ionization, ESI-)和多反应监测模式(multiple reaction monitoring, MRM)检测。结果 在0~20.0 μg/L范围内, 14种目标化合物呈良好的线性关系, 相关系数(r2)大于0.99, 方法的定量限(S/N=10)为0.433.0 μg/L; 14种黄酮类化合物在10.0、50.0和100.0 μg/L 3个水平下的平均加标回收率为78.8%~96.7%, 相对标准偏差(RSD, n=6)为2.8%~15.1%。结论 该方法简单、高效、灵敏、准确, 可用于同时测定保健酒中14种黄酮类成分的含量。

英文摘要:

      Objective To establish a method for simultaneous determination of 14kinds of flavones in health-care wine by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was diluted with acetonitrile-water (2:8, V:V) for 10 times. The separation was performed on a Luna?C8(2) column(100A, 150 mm×2 mm, 3 μm) using a mobile phase gradient with 0.1% formic acid and acetonitrile. The determination was carried out with electrospray ionsource under the negative mode with multiple-reaction monitoring (MRM) mode. Results This method had good linear relationships with correlation coefficients (r2) not less than 0.99 in the range of 020.0 μg/L. The limits of quantification (LOQ, S/N=10) of 14kinds of flavones were varied from 0.4 μg/L to 33.0 μg/L. At the spiked levels of 10.0, 50.0 and 100.0 μg/L, the average recoveries of the 14kinds of flavones ranged from 78.8% to 96.7%, and the relative standard deviation (RSD, n=6) was 2.8%15.1%. Conclusion This method is simple, efficient, sensitive, and accurate, and can be used to detect the 14 kinds of flavones in health-care wine.