矿泉水中铜绿假单胞菌的测定能力验证结果分析

Proficiency testing results analysis of determination of Pseudomonas aeruginosa in mineral water

中文关键词:  能力验证  铜绿假单胞菌  VITEK2  矿泉水

英文关键词:proficiency testing  Pseudomonas aeruginosa  VITEK2  mineral water

中文摘要:

      目的 分析矿泉水中铜绿假单胞菌检测能力验证(ACAS-PT 736(2019))的结果。方法 根据能力验证指导书, 并参照GB 4789.2-2016《食品安全国家标准食品微生物学检验 菌落总数测定》对样品进行稀释, 依据GB 8538-2016《食品安全国家标准 饮用天然矿泉水检验方法》对可疑菌落进行鉴定, 同时对可疑菌落使用VITEK2进行辅助鉴定。结果 样品19-P383检测结果为7800 CFU/250 mL, 样品19-Q333检测结果为8800 CFU/ 250 mL。本次能力验证Z值分别为?0.8、?0.1、|Z|均小于2, 检测结果满意。结论 本实验室具备检测铜绿假单胞菌的能力, VITEK2对可疑菌落进行辅助鉴定的准确性也得到了验证。

英文摘要:

      Objective To analyze the results of the proficiency testing (ACAS-PT 736 (2019) of Pseudomonas aeruginosa in mineral water. Methods Samples were diluted according to the proficiency test guidance and GB 4789.2-2016 National food safety standard-Food microbiological inspection total colony determination. Suspicious colonies were identified according to GB 8538-2016 National food safety standard-Test method for drinking natural mineral water, and VITEK2 was used for auxiliary identification of suspicious colonies at the same time. Results The test result of sample 19-P383 was 7800 CFU/250 mL, and the test result of sample 19-Q333 was 8800 CFU/ 250 mL. The test results were satisfactory as the Z values of the proficiency test were ?0.8, ?0.1, and |Z| were less than 2. Conclusion This laboratory has the ability to detect Pseudomonas aeruginosa in mineral water, and the accuracy of VITEK2' s auxiliary identification of suspicious colonies has been verified.