气相色谱法测定南瓜中肌醇的含量

Determination of inositol in pumpkin by gas chromatography

中文关键词:  气相色谱法  肌醇  南瓜

英文关键词:gas chromatography  inositol  pumpkin

中文摘要:

      目的 建立气相色谱检测法测定南瓜中肌醇的方法。方法 样品粉碎匀浆后, 经水+无水乙醇(15:35, V:V)超声提取20 min, 涡旋振荡25 min浓缩烘干, 复溶后进行衍生化。用氢火焰离子化检测器检测, 外标法定量。结果 肌醇衍生物在10~200 mg/L范围内线性关系良好, 相关系数r2为0.99996。检出限与定量限分别为1.0、3.0 mg/kg, 回收率为88.5%~100.3%, 相对标准偏差小于5%, 使用上述方法对23批次南瓜样品进行肌醇含量检测, 结果为784~1792 mg/kg。结论 该方法高效, 便捷, 准确度高, 适用于南瓜等各类蔬菜水果中肌醇的检测。

英文摘要:

      Objective To establish a method for the determination of inositol in pumpkin by gas chromatography. Methods After the sample was crushed and homogenized, it was extracted with water and absolute ethanol (15:35, V:V) ultrasonic for 20 min, vortexed for 25 min, concentrated and dried, and derivatized after redissolving. It is detected by hydrogen flame ionization detector and quantified by external standard method. Result The inositol derivative had a good linear relationship in the range of 10–200 mg/L, and the correlation coefficient r2 was 0.99996. The limit of detection and the limit of quantification were 1.0 mg/kg and 3.0 mg/kg, respectively. The recovery rates were 88.5%–100.3%, and the relative standard deviation was less than 5%. The inositol content of 23 batches of pumpkin samples was tested and the results were 784–1792 mg/kg. Conclusion This method is efficient, convenient, and accurate for determination of inositol in pumpkin as well as other fruits and vegetables.