2种高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的不确定度评定

Uncertainty evaluation for the determination of aflatoxin B1 in sesame sauce by 2 kinds of high performance liquid chromatograph

中文关键词:  黄曲霉毒素B1  高效液相色谱法  不确定度

英文关键词:aflatoxin B1  high performance liquid chromatography  uncertainty

中文摘要:

      目的 评定并比较2种厂家高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的不确定度。方法 分别采用Agilent1260型及Thermo U3000型高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的含量, 应用不确定度理论分析检测数学模型, 对测定不确定度的来源进行分析和量化。结果 结果表明当黄曲霉毒素B1的测定结果(Agilent 1260、Thermo U3000)分别为6.1236、6.0230 μg/kg时, 其扩展不确定度分别为0.42706、0.31115 μg/kg, k=2。结论 标准溶液的配制、由最小二乘法拟合标准曲线计算样品浓度以及回收率测定引入的不确定度对结果影响较大。在本实验条件下, Agilent1260型测定引入的相对不确定度大于Thermo U3000型测定引入的不确定度, 后者仪器更加稳定, 结果更准确。

英文摘要:

      Objective To evaluate and compare the difference of uncertainty of determination of aflatoxin B1 in sesame sauce by 2 kinds of high performance liquid chromatograph. Methods The content of aflatoxin B1 in sesame sauce was determined by Agilent 1260 and Thermo U3000 high performance liquid chromatography, and the mathematical model of uncertainty analysis was used to analyze and quantify the sources of measurement uncertainty. Results The results (Agilent 1260, Thermo U3000) showed when the determination results of aflatoxin B1 were 6.1236 μg/kg and 6.0230 μg/kg, the extended uncertainties were 0.42706 μg/kg, 0.31115 μg/kg, and k=2. Conclusion The uncertainty introduced by the preparation of standard solution, the calculation of sample concentration by the least square method fitting standard curve and the determination of recovery has great influence on the results. Under this experimental condition, the relative uncertainty introduced by the Agilent 1260 measurement is greater than that introduced by the Thermo U3000 measurement, which is more stable and more accurate.