高效液相色谱法测定蜂胶软胶囊中白杨素和高良姜素的含量

Determination of chrysin and galangin content in propolis soft capsules by high performance liquid chromatography

中文关键词:  蜂胶  白杨素  高良姜素  高效液相色谱法

英文关键词:propolis  chrysin  galangin  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定保健食品蜂胶软胶囊中白杨素和高良姜素含量的方法。方法 样品经乙醇提取后, 由C18色谱柱分离, 以甲醇-0.1%磷酸(60:40, V:V)为流动相进行等度洗脱, 用紫外检测器在波长为270 nm检测, 外标法定量。结果 白杨素在5.685~284.25 μg/mL浓度范围内线性关系良好, 相关系数为0.999, 加标回收率为97.1%~103.0%, 相对标准偏差(relative standard deviation, RSD)为0.8%~1.8%; 高良姜素在5.32~266 μg/mL浓度范围内线性关系良好, 相关系数为0.999, 加标回收率为96.2%~102.0%, RSD为0.5%~1.9%。结论 此方法简便快速, 准确可靠, 可提高保健食品蜂胶软胶囊中白杨素和高良姜素含量测定的效率。

英文摘要:

      Objective To establish a method for the determination of chrysin and galangin content in health food propolis soft capsules by high performance liquid chromatography. Methods The sample was extracted with ethanol, separated on a C18 column and isocratic eluted with methanol-0.1% phosphoric acid (60:40, V:V) as mobile phase. The targets were detected by a ultraviolet detector at the wavelength of 270 nm, and quantified by external standard method. Results Chrysin had a good linear relationship in the concentration range of 5.685?284.25 μg/mL, and the correlation coefficient was 0.999. The spiked recoveries ranged from 97.1%?103.0% with the standard deviations of 0.8%?1.8%. Galangin had a good linear relationship in the concentration range of 5.32~266 μg/mL, and the correlation coefficient was 0.999. The spiked recoveries ranged from 96.2%?102.0% with the standard deviations of 0.5%?1.9%. Conclusion This method is simple, rapid, accurate and reliable, and can improve the efficiency of the determination of chrysin and galangin in propolis soft capsule of health food.