辣椒制品中罗丹明B胶体金免疫层析法的建立

Development of a colloidal gold-based immunochromatographic assay for the detection of rhodamine B in chili products

中文关键词:  罗丹明B  胶体金免疫层析技术  辣椒制品  检测卡

英文关键词:rhodamine B  colloidal gold immunochromatography assay  chili products  strip

中文摘要:

      目的 建立灵敏的胶体金免疫层析法快速检测辣椒制品中的罗丹明B (rhodamine B, RB)。方法 用柠檬酸三钠还原法制备胶体金, 并通过物理吸附标记罗丹明B单克隆抗体。在优化条件下, 建立了基于竞争性抑制的罗丹明B的可视化胶体金免疫层析法。结果 使用建立的方法检测辣椒制品中罗丹明B, 定性检测限为5 μg/kg, 满足了国家安全限量要求; 对实际样本的检测结果与液质联用检测结果一致。结论 本研究建立的方法可以实现对辣椒制品中非法使用罗丹明B的灵敏、快速、高效的检测。

英文摘要:

      Objective To establish a sensitive colloidal gold immunochromatography method for the rapid detection of rhodamine B (RB) in pepper products. Methods Colloidal gold was prepared by sodium citrate reduction method, and it was labeled with RB monoclonal antibody by physical adsorption method. Under optimized conditions, a sensitive colloidal gold immunochromatography (GICA) was developed based on competitive binding immunoassay. Results RB in pepper products was detected by the established method, and the qualitative detection limit was 5 μg/kg, which met the requirements of national security limits. The results of the actual samples by this method were consistent with those of the liquid chromatography-mass spectrometry (LC-MS) method. Conclusion The established GICA is sensitive, rapid and efficient for the illegal use of RB residues in chili products.