我国部分地区燕麦及其制品中柄曲霉素污染调查

Investigation on contamination of sterigmatocystin in oat and products in some areas of China

中文关键词:  燕麦  燕麦粉  燕麦片  柄曲霉素  高效液相色谱串联质谱法

英文关键词:oat  oat flour  oatmeal  sterigmatocystin  high performance liquid chromatography-mass spectrometry

中文摘要:

      目的 建立燕麦及其制品中柄曲霉素(sterigmatocystin, STC)检测方法, 并了解我国部分地区燕麦及其制品中STC的污染情况。方法 在内蒙古农户家中采集新产燕麦136份, 同时采集内蒙古超市中散装燕麦粉5份以及我国大陆地区市售燕麦片96份。采用QuEChERS (quick, easy, cheap, effective, rugged, safe)前处理结合高效液相色谱串联质谱(high performance liquid chromatography-mass spectrometry, HPLC-MS/MS)方法检测STC的含量。结果 在燕麦和燕麦片中分别添加低、中、高浓度的STC, 其平均回收率范围为83.6%~119.4%, RSD为2.3%~15.4%, 方法定量限为0.5 μg/kg, 在燕麦和燕麦片中的检出限分别为0.02 μg/kg和0.05 μg/kg, 表明该方法可用于燕麦和燕麦片中STC含量的检测。分析检测结果可知燕麦、燕麦粉和燕麦片3种样品中均检出较低浓度的STC, 检出率分别为0.7%、60%和13.5%, 平均含量分别为0.1、0.2和 0.11 μg/kg。结论 内蒙古地区燕麦、燕麦粉和我国大陆地区市售燕麦片中柄曲霉素污染水平整体较低, 但在监管中仍不容忽视, 该研究同时也为以后开展居民膳食中STC风险评估提供基础数据。

英文摘要:

      Objective To establish the detection method of sterigmatocystin (STC) in oat and their products, and to investigate the contamination of STC in oat in some areas of China. Methods The 136 samples of oat in present year were collected from the households, 5 oat flour were collected from supermarket in Inner Mongolia, and 96 oat meals were purchased from supermarket in some areas of mainland China. The content of STC was analyzed using methods based on QuEChERS (quick, easy, cheap, effective, rugged, safe) pre-treated with liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results The oat and oatmeal was spiked with low, medium and high concentration of STC, the average recoveries were in the range of 83.6%119.4%. RSD was 2.3%15.4%, The limit of quantification (LOQ) was 0.5 μg/kg, limits of detection (LOD) were 0.02 μg/kg and 0.05 μg/kg for oat and oatmeal, respectively. This means that the developed method can be completely used to detect STC in oat and oatmeal. Analytical detected results showed that the STC was occurrence in three items at a lower concentration, the detection rate of STC in oat, oat flour and oatmeal was 0.7%, 60% and 13.5%, and the average content was 0.1, 0.2 and 0.11 μg/kg, respectively. Conclusion The contamination levels of STC in oat and oat flour from Inner Mongolia and oatmeal from markets in mainland China are overall low, but can not be ignored in supervise, meanwhile, this study also provides the basic data for the risk assessment of STC in the future.