高效液相色谱法测定软糖中的胭脂红酸

Determination of carminic acid in soft sweets by high performance liquid chromatography

中文关键词:  软糖  胭脂红酸  高效液相色谱法

英文关键词:soft sweets  carminic acid  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定软糖中胭脂红酸含量的方法。方法 通过加热溶解软糖释放出其中的胭脂红酸, 再采取过聚酰胺粉层析柱, 进行除杂、洗脱、净化, 采取磷酸二氢钾溶液-乙腈作为流动相, 进行梯度洗脱, 经C18色谱柱(4.6 mm×250 mm, 5.0 μm); 流速: 1.0 mL/min; 柱温: 35 ℃; 进样量: 10 μL; 检测波长: 494 nm; 样品分析时间30 min。结果 胭脂红酸线性范围为0.4~4.0 μg/mL, 方法相关性好(r2=0.9999), 方法检出限为0.6 mg/kg, 定量限为1.6 mg/kg, 相对标准系数(relative standard deviation, RSD)为2.9%, 回收率范围是99.70%~109.51%(n=9)。结论 此法操作方便、准确、灵敏, 适合测定软糖中的胭脂红酸, 能有效地对软糖中添加胭脂红酸的量进行控制。

英文摘要:

      Objective To establish a high performance liquid chromatography(HPLC) method for the determination of carminic acid in soft sweets. Methods The carminic acid in soft sweets was released by heating and dissolving the soft sugar, and then it was purified and condensed by polyamide powder chromatography column. Potassium dihydrogen phosphate solution-acetonitrile was used as mobile phase for gradient elution by C18 chromatography column (4.6 mm×250 mm, 5.0 μm), with flow rate 1.0 mL/min, column temperature 35 ℃, sample volume 10 μL, detection wavelength 494 nm and analysis time 30 min. Results The linear relation of the method was good with r2=0.9999 in linear range of carminic acid at 0.4-4.0 μg/mL. The detection limit was 0.6 mg/kg, the quantitative limit was 1.6 mg/kg, the RSD(relative standard deviation) was 2.9%, and the recovery rate was 99.70%-109.51%(n=9). Conclusion This method is convenient, accurate and sensitive, which is suitable for the determination of carminic acid in soft sweets and can effectively control the amount of carminic acid added in soft sweets.