不同粒度对三七粉主要有效成分体外溶出度的影响

Effects of different particle sizes on the dissolution of main effective components in Panax notoginseng powder in vitro

中文关键词:  三七粉  体外溶出度  高效液相色谱法  溶出曲线  有效成分

英文关键词:Panax notoginseng powder  dissolution in vitro  high performance liquid chromatography  dissolution curve  effective components

中文摘要:

      目的 分析不同粒度对三七粉末主要有效成分在体外溶出度方面的影响。方法 采用高效液相色谱法法测定不同粒度三七粉末, 包括粗粉(24~65目)、中粉(65~80目)和细粉(80~200目), 在磷酸缓冲液、水和醋酸缓冲液中主要有效成分的溶出量, 并绘制相应的溶出曲线以分析影响。结果 在不同溶出介质中三七中粉和细粉在短时间内可以达到溶出平衡, 而三七粗粉在30 min才达到溶出平衡; 在3种溶出介质中三七中粉累积溶出度均为最大; 在不同介质中三七总皂苷溶出率为: pH6.8的磷酸缓冲溶液>水(pH=5.13)>pH4.5的醋酸缓冲溶液。结论 建议三七粉碎入药时, 磨成中粉进行使用。

英文摘要:

      Objective To analyze the effects of different particle sizes on the dissolution of main effective components in Panax notoginseng powder in vitro. Methods High performance liquid chromatography(HPLC) method was used to determine the dissolution amount of main effective components in Panax notoginseng powder with different particle sizes including coarse powder (24-65 mesh), medium powder (65-80 mesh) and fine powder (80-200 mesh) in phosphoric acid buffers, water buffers, and acetic acid buffers, and the corresponding dissolution curve was drawn to analyze the effects. Results In different dissolution media, the medium and fine Panax notoginseng particles could reach dissolution equilibrium in a short time while the coarse Panax notoginseng powder reached dissolution equilibrium in 30 min. In the 3 dissolution media, the cumulative dissolution percentage of the middle Panax notoginseng particles was highest. In different dissolution media, the order of accumulated dissolved ratio was: pH6.8 phosphate buffer solution > water (pH=5.13) >pH4.5 acetic acid buffer solution. Conclusion It is recommended that when Panax notoginseng is chosen as medicine, it should be crushed to medium particles.