高效液相色谱-串联质谱法同时测定食品接触材料中11种双酚A类和3种烷基酚类化合物迁移量

Determination of migration amount of 11 bisphenol A and 3 alkyl phenol compounds in food contact materials by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱  食品接触材料  酚类物质  迁移量

英文关键词:high performance liquid chromatography tandem mass spectrometry  food contact materials  phenolics  migration amount

中文摘要:

      目的 建立高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry, LC-MS /MS)法同时测定食品接触材料中11种双酚类及3种烷基酚类化合物。方法 14种目标化合物在塑料制品中通过与食品模拟物在一定温度、时间接触的状态下迁移到食品模拟物中, 以T3色谱柱进行分离, 甲醇: 乙腈(1:1, V:V)-5 mmol/L乙酸铵水为流动相洗脱, 采用电喷雾离子源(electrospray ionization, ESI), 多反应监测(multiple reaction monitoring, MRM) 负离子模式进行扫描测定。结果 14种酚类化合物的色谱分离良好, 1~200 μg/L质量浓度范围内与其峰面积均呈线性关系(双酚S为0.2~40.0 μg/L), 且线性关系良好(r2≥0.995)。方法检出限为0.01~0.78 μg/L; 加标回收率范围为84.5%~99.2%, 相对标准偏差(relative standard deviation, RSD)为0.7%~12.4%。结论 该方法快速可靠、准确简便, 适用于食品接触材料中双酚A类化合物和烷基酚类化合物的检测。

英文摘要:

      Objective To establish a high performance liquid chromatography-tandem mass spectarometry (LC-MS/MS)method for the determination of migration amount of 11 bisphenol and 3 alkyl phenol compounds in food contact materials. Methods 11 bisphenol and 3 alkyl phenol compounds transferred into food simulants in plastic products through contacting with the food simulants at a certain temperature and time. The separation was performed using a T3 chromatographic column with methanol: acetonitrile (1:1, V:V)-5 mmol/L ammonium acetate solution as mobile phase. Under the electrospray ionization (ESI) mode, 11 bisphenol analogues and 3 alkyl phenol compounds were detected in a multiple reaction monitoring(MRM) mode. Results The method indicated a perfect performance in chromatographic separation. The calibration curves of 11 bisphenol and 3 alkyl phenol compounds were linear in the range of 1200 μg/L(0.2-40.0 μg/L for bisphenol S) with a good linear relationship (r2≥0.995) and the limits of detection was 0.01-0.78 μg/L. The recoveries ranged from 84.5% to 99.2%, with relative standard deviation(RSD) 0.7%~12.4%. Conclusion This method is rapid, accurate, precise and convenient, and is appropriate for the determination of bisphenol A and alkyl phenol compounds in food contact materials.