罗非鱼中菌落总数测量结果的不确定度评定

Evaluation of uncertainty in measurement results of aerobic plate count in tilapia

中文关键词:  菌落总数  罗非鱼  不确定度

英文关键词:aerobic plate count  tilapia  uncertainty

中文摘要:

      目的 对罗非鱼中菌落总数测量结果进行不确定度评定。方法 采用平板计数法测定罗非鱼中的菌落总数, 通过建立数学模型, 识别不确定度来源, 对不确定度来源进行分析, 估算出各不确定度分量对测量不确定度的影响, 最终合成测量结果的相对标准不确定度。结果 被测样品中细菌总数为23000 CFU/g, 在95%的置信区间下, 扩展不确定度为920 CFU/g(k=2)。结论 样品重复测定和结果修约是影响该方法不确定度的主要因素, 因此该方法测量不确定度的评定对检测结果准确度的提高具有指导意义。

英文摘要:

      Objective To evaluate the uncertainty of the total bacterial colony in tilapia. Methods The total number of colonies in tilapia was determined by plate counting method, the source of uncertainty was identified by establishing mathematical model, the source of uncertainty was analyzed, the influence of each uncertainty component on the measurement uncertainty was estimated, and the relative standard uncertainty of the measurement results was finally synthesized. Results The total number of bacteria in the samples was 23,000 CFU/g, and the extended uncertainty was 920 CFU/g (k=2) with a 95% confidence interval. Conclusion The main factors affecting the uncertainty of this method are the repeated determination of samples and the modification of results. Therefore, the evaluation of the uncertainty of the method is of guiding significance for the improvement of the accuracy of the test results.