QuEChERS-超高效液相色谱-串联质谱法同时测定蔬菜中30种农药残留

Simultaneous detection of 30 pesticide residues in vegetables by QuEChERS–ultra performance liquid chromatography–tandem mass spectrometry

中文关键词:  QuEChERS  超高效液相色谱-串联质谱法  农药多残留  同时测定  蔬菜

英文关键词:QuEChERS  ultra performance liquid chromatography-tandem mass spectrometry  pestcide residues  simultaneous detection  vegetables

中文摘要:

      目的 应用超高效液相色谱-串联质谱法同时测定蔬菜中30种农药残留。方法 蔬菜样品经粉碎后, 乙腈超声提取, 采用简化的QuEChERS法净化, 超高效液相色谱-串联质谱仪检测。结果 30种农药在相应浓度范围内线性良好, 相关系数r为0.9991~1.0000, 在所添加的3个水平浓度下, 方法平均回收率为70.6%~102.6%, 相对标准偏差为0.3%~9.6%。结论 方法操作简便, 灵敏度高, 分离效果好, 回收率高, 30种农药的检出下限均低于国家标准, 可应用于实际蔬菜样品中多农药残留的同时检测。

英文摘要:

      Objective To establish a method for simultaneous determination of 30 pesticide residues in vegetables by ultra performance liquid chromatography-tandem mass spectrometry. Methods After crushing, the vegetable samples were extracted by ultrasonic extraction of acetonitrile, purified by simplified QuEChERS method, and detected by ultra performance liquid chromatography- mass spectrometer. Results The 30 pesticides showed good linearity in the corresponding concentration range, and the regression coefficient r was 0.99911.0000. Under the 3 added horizontal concentrations, the average recovery was 70.6%102.6%, and the relative standard deviation was 0.3%9.6%. Conclusions This method is simple to operate, high sensitivity, good separation effect and high recovery rate. The lower detection limit of 30 kinds of pesticides is lower than the national standard.