评定高效液相色谱法测定婴幼儿配方乳粉中乳铁蛋白含量的不确定度

Uncertainty evaluation of determination of lactoferrin in infant formula milk powder by high performance liquid chromatography

中文关键词:  婴幼儿配方乳粉  乳铁蛋白  不确定度  高效液相色谱

英文关键词:infant formula milk powder  lactoferrin  uncertainty evaluation  high performance liquid chromatography

中文摘要:

      目的 对高效液相色谱法测定婴幼儿配方乳粉中乳铁蛋白含量的不确定度进行分析。方法 通过对实验过程中所用标准物质、样品处理过程、测量重复性以及回收率4个方面进行不确定度分析, 建立了不确定度的模型, 计算合成不确定度、扩展不确定度。结果 当乳铁蛋白的含量为64.9 mg/100 g时, 高效液相色谱法测定婴幼儿乳粉中乳铁蛋白含量的合成不确定度为0.023, 扩展不确定度为1.5 mg/100 g, 乳粉中乳铁蛋白含量的测定结果为(64.9±1.5) mg/100 g, (k=2, P=95%)。结论 本次评定揭示了该检测方法对结果影响最大的因素是标准溶液及工作液的制备过程, 因此在此过程中应严格按规范进行操作, 从而使检测结果更加准确可靠。

英文摘要:

      Objective To analyze the uncertainty of determination of lactoferrin in infant formula milk powder by high performance liquid chromatography (HPLC). Methods By analysing the uncertainty of the standard materials used in the experiment, sample processing, measurement repeatability and recovery rate, the uncertainty model was established to calculate the combined uncertainty and expanded uncertainty. Results When the content of lactoferrin was 64.9 mg/100 g, the synthetic uncertainty of lactoferrin in milk powder was 0.023, the extended uncertainty was 1.5 mg/100 g, and the content of lactoferrin in milk powder was (64.9±1.5) mg/100 g, (k=2, P=95%). Conclusion This evaluation reveals that the preparation process of standard solution and working solution is the most influential factor of the test method on the results. Therefore, specification should be strictly followed during the operation procedure to improve the reliability of the test results.