高效液相色谱-串联质谱法同时测定饮料中54种食品添加剂

Simultaneous determination of 54 food additives in beverages by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱法  食品添加剂  饮料  筛查

英文关键词:high performance liquid chromatography-tandem mass spectrometry  additives  beverage  screening

中文摘要:

      目的 建立高效液相色谱-串联质谱法同时测定饮料中的11种甜味剂, 33种着色剂，7种防腐剂和3种抗氧化剂的分析方法。方法 样品经50%甲醇水混合溶液提取后, 采用Atlantis T3色谱柱分离, 以不同比例的10 mmol·L-1乙酸铵水溶液和乙腈作为流动相进行梯度洗脱。采用电喷雾正负离子模式和多反应监测模式进行测定。结果 54种待测物质的质量浓度在一定范围内与峰面积呈线性关系, 相关系数大于0.990, 定量限为71.1~1323 μg·kg-1。加标回收率为73.5%～117.6%, 相对标准偏差为0.4%～16.7%。结论 该方法快速、准确、灵敏度高, 可用于饮料中多种添加剂的测定。

英文摘要:

      Objective To establish a method for simultaneous determination of 11 sweeteners, 33 pigments ,7 preservatives and 3 antioxidants in beverages by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The samples were extracted by a mixture of 50% methanol and water, then separated by Atlantis T3 column. The 10 mmol·L-1 ammonium acetate solution and acetonitrile were used as mobile phase for gradient elution, and then the samples were detected by electrospray positive and negative ion mode and multi-reaction monitoring mode. Results The mass concentration of 54 kinds of test substances had a linear relationship with the peak area in a certain range, and the correlation coefficients were greater than 0.990. The limit of quantitation was 71.1-1323 μg·kg-1. The standard recoveries were 73.5%-117.6%, and the relative standard deviations were 0.4%-16.7%. Conclusion The method is rapid, accurate and sensitive, and it can be applied for the determination of various additives in beverages.