高效液相色谱法测定磷脂软胶囊中磷脂酰胆碱、磷脂酰肌醇、磷脂酰乙醇胺的含量

Determination of phosphatidyl cholines, phosphatidyl ethanolamine and phosphatidylinositol in phospholipid soft capsules by high performance liquid chromatography

中文关键词:  磷脂酰胆碱  磷脂酰乙醇胺  磷脂酰肌醇  高效液相色谱  快速检测

英文关键词:phosphatidyl cholines  phosphatidyl ethanolamine  phosphatidylinositol  high performance liquid chromatography  rapid detection

中文摘要:

      目的 建立高效液相色谱法同时检测磷脂软胶囊中磷脂酰胆碱(phosphatidyl cholines, PC), 磷脂酰乙醇胺(phosphatidyl ethanolamine, PE), 磷脂酰肌醇(phosphatidylinositol, PI)3种组分的含量。方法 样品用甲醇超声提取后, 经Bridge? HILIC色谱柱(4.6 mm×150 mm, 5 μm)分离, 以0.9 mmol/L甲酸铵溶液-乙腈为流动相进行梯度洗脱, 流速为1.0 mL/min; 柱温30 ℃; 检测波长为205 nm, 外标法定量。结果 磷脂酰胆碱, 磷脂酰乙醇胺, 磷脂酰肌醇在浓度0.025~0.250 mg/mL范围内线性关系良好, 相关系数均大于0.99, 检出限分为0.300~1.386 mg/g, 定量限为1.002~4.624 mg/g, 加标回收率分别为90%~105%, 相对标准偏差为1.0%~4.0%。结论 该方法高效, 便捷, 准确度高, 适用于磷脂软胶囊中3种磷脂成分的同时检测。

英文摘要:

      Objective To establish a method for the simultaneous determination of phosphatidyl cholines (PC), phosphatidyl ethanolamine (PE) and phosphatidylinositol (PI) in phospholipid soft capsules by high performance liquid chromatography. Methods After ultrasonic extraction with methanol, the samples were separated with a Bridge? HILIC column (4.6 mm×150 mm, 5 μm) at the flow rate of 1.0 mL/min by gradient elution using 0.9 mmol/L ammonium formate solution-acetonitrile as mobile phase, the column temperature was 30 ℃, the detection wavelength was 205 nm. The contents were quantitatively analyzed by external standard method. Results Phosphatidyl cholines, phosphatidyl ethanolamine and phosphatidylinositol had good linear relationships in the range of 0.025?0.250 mg/mL, and the correlation coefficients were more than 0.99. The limits of detection were 0.300?1.386 mg/g and the limits of quantitation were 1.002?4.624 mg/g. The recoveries were 90%?105%, with the relative standard deviations of 1.0%?4.0%. Conclusion This method is efficient, convenient, and accurate, and is suitable for the simultaneous detection of 3 phospholipid components in phospholipid soft capsules.