气相色谱法测定婴幼儿乳粉中的1,3-二油酸-2-棕榈酸甘油三酯

Determination of 1,3-dioleate-2-palmitic triglyceride in infant formula milk powder by gas chromatography

中文关键词:  高效气相色谱  氢离子火焰检测器  婴幼儿配方乳粉  1,3-二油酸-2-棕榈酸甘油三酯

英文关键词:gas chromatography  hydrogen flame ionization detector  infant formula milk powder  1,3-dioleate- 2-palmitic triglyceride

中文摘要:

      目的 建立气相色谱检测方法测定婴幼儿配方乳粉中1,3-二油酸-2-棕榈酸甘油三酯的含量。方法 乳粉中的油脂经过石油醚和乙醚提取后, 离心机分层, 用正己烷溶解, 并过滤膜后, 经气相色谱仪氢火焰离子化检测器检测, 外标法定量测定乳粉中1,3-二油酸-2-棕榈酸甘油三酯的含量。结果 得到的检测峰型良好, 在线性范围0.10~5.00 mg/mL内, 线性关系良好, 相关系数r2大于0.995。回收率在90%~104%之间, 重复测定20次, 相对标准偏差0.0692%, 结论 该方法快速, 灵敏, 准确, 适合测定婴幼儿配方乳粉中1,3-二油酸-2-棕榈酸甘油三酯的含量。

英文摘要:

      Objective To establish a method for the determination of 1,3-dioleate-2-palmitic triglyceride in infant formula milk powder by high performance gas chromatography. Methods After the grease in milk powder was extracted by petroleum ether and ethyl ether extract, it was layered in a centrifuge and dissolved by with n-hexane. And after the membrane filtration, the content of 1,3-dioleate-2-palmitic triglyceride was determined by gas chromatography with hydrogen flame ionization detector and quantified by external standard method. Results The detection peak was good and in the linear range of 0.105.00 mg/mL, the liner relation was good with the liner coefficient r2 over 0.995. The recovery rate was between 90% and 104% and the relative standard deviation of 20 times repeated determination was 0.0692%. Conclusion This method is rapid, sensitive, accurate, which is suitable for the determination of 1,3-dioleate-2-palmitic triglyceride in infant formula milk powder.