超高效液相色谱法同时测定饮料中20种食品添加剂

Simultaneous determination of 20 food additives in beverages by ultra performance liquid chromatography

中文关键词:  食品添加剂  超高效液相色谱法  饮料  同时检测  非法添加

英文关键词:food additive  ultra performance liquid chromatography  beverage  simultaneous determination  illegal additive

中文摘要:

      目的 建立超高效液相色谱法同时检测饮料中的20种食品添加剂的分析方法。方法 使用waters XBridge C18(5 μm, 4.6 mm×250 mm)色谱柱, 用0.01 mol/L 磷酸二氢钾(pH 4.8-5.0)和乙腈为流动相进行梯度洗脱, 在35 ℃柱温, 1.0 mL/min流速下, 采用光电二极管阵列(photo-diode array, PDA)检测器在210、230 nm波长处进行检测, 外标法定量, 15 min内可实现20种食品添加剂的分离。结果 20种添加剂均具有良好线性, 相关系数均大于0.999, 检出限0.3~10 mg/kg, 平均回收率为81.50%~110.20%, 相对标准偏差小于5% (n=8)。 结论 该方法灵敏、准确, 适用于饮料中20种添加剂的同时检测。

英文摘要:

      Objective To establish a method for the simultaneous determination of 20 kinds of additives in beverages by ultra performance liquid chromatography. Methods The separation was achieved on a XBridge C18 column(5 μm, 4.6 mm×250 mm)in 15 min by using 0.01 mol/L KH2PO4(pH 4.8?5.0 and acetonitrile as mobile phase with a flow rate of 1.0 mL/min at 35 ℃. A diode array detector was used to detect at 210, 230 nm. The quantification was performed by the external standard method. Results All the 20 additives had good linearity, and the correlation coefficients were all greater than 0.999. The limits of detection were 0.3?10 mg/kg, average recovery rates were 81.50%?110.20%, and RSDs were less than 5% (n=8). Conclusion This method is sensitive and accurate and is suitable for simultaneous detection of 20 kinds of additives in beverages.