QuEChERS结合液相色谱-串联质谱法检测茶叶中34种农药残留

Determination of 34 kinds of pesticide residues in tea by QuEChERS combined with liquid chromatography tandem mass spectrometry

中文关键词:  QuEChERS  液相色谱-串联质谱法  茶叶  农药残留

英文关键词:QuEChERS  performance liquid chromatography-tandem mass spectrometry  tea  pesticide residue

中文摘要:

      目的 建立QuEChERS结合液相色谱-串联质谱法同时测定茶叶中34种农药残留的分析方法。 方法 样品加入乙腈后使用漩涡混合和超声波提取, 应用QuEChERS方法进行净化处理。采用0.05%甲酸水(A)和乙腈(B)作为流动相进行梯度洗脱, 质谱(ESI+)采用多反应离子监测模式对34种农药的定量离子和定性离子进行监测。结果 本方法在17 min内完成34种目标化合物的分离分析。34种农药在2.5~200 μg/L范围内线性关系良好, 相关系数(r)均大于0.995, 方法的定量限为0.01~5.11 μg/kg。34种农药在20、50和100 μg/kg添加水平的回收率为70.6%~116.1%, 相对标准偏差为1.0%~8.1% (n=6)。结论 该方法简单、快速、准确、灵敏高, 适合用于茶叶中农药多残留的测定。

英文摘要:

      Objective To establish a method for the simultaneous determination of 34 pesticide residues in tea by QuEChERS combined with liquid chromatography-tandem mass spectrometry. Methods After adding acetonitrile, the samples were extracted by vortex mixing and ultrasonic wave. The 0.05% formic acid water (A) and acetonitrile (B) were used as mobile phases for gradient elution, and the quantitative and qualitative ions of 34 pesticides were monitored by mass spectrometry (ESI+) in A multi-reactive ion monitoring mode. Results This method completed the separation analysis of 34 target compounds in 17 min. The linear relationship of 34 pesticides was good in the range of 2.5?200 μg/L, the correlation coefficients were greater than 0.995, and the limit of quantification were 0.01?5.11 μg/kg. The recoveries of 34 pesticides at 20, 50 and 100 μg/kg levels ranged from 70.6% to 116.1%, and the relative standard deviation was 1.0%?8.1% (n=6). Conclusion This method is simple, fast, accurate and sensitive, which is suitable for detecting 34 pesticide residues in tea.