响应面法优化超声辅助竹叶鸡爪茶多酚的提取工艺

Optimization of ultrasonic assisted extraction process of polyphenols from Rubus bambusarum Focke by response surface methodology

中文关键词:  竹叶鸡爪茶  多酚  超声波辅助提取  响应面

英文关键词:Rubus bambusarum Focke  polyphenols, ultrasonic assisted extraction  response surface

中文摘要:

      目的 采用响应面法优化竹叶鸡爪茶多酚的超声提取工艺。 方法 通过超声辅助法提取竹叶鸡爪茶中的总酚, 以总酚含量为评价指标, 考察超声温度、超声功率、超声时间、乙醇浓度、液料比5个因素对总酚含量的影响, 选择4个因素(超声温度、超声时间、乙醇浓度、液料比)设计4因素3水平Box-Behnken响应面试验进行工艺优化。结果 竹叶鸡爪茶多酚的最佳提取工艺为: 超声温度71 ℃、超声时间35 min、液料比42:1 (V:m)、乙醇浓度57%, 此条件下测得的多酚含量(2.33±0.01) mg/g, 与预测值2.37 mg/g接近, 相对误差为1.68%, 表明模型预测值与实际值基本吻合。结论 超声辅助提取法能够有效地提取竹叶鸡爪茶多酚。

英文摘要:

      Objective To optimize ultrasonic assisted extraction process of polyphenols from Rubus bambusarum Focke by response surface methodology. Methods Total phenol from Rubus bambusarum Focke was extracted by ultrasound-assisted method. Taking the total phenol content as the evaluation index, the effects of ultrasonic temperature, ultrasonic power, ultrasonic time, ethanol concentration and liquid-to-liquid ratio on the total phenol content were investigated. The 4 factors (ultrasound temperature, ultrasonic time, ethanol concentration, liquid to material ratio) were selected to design a 4-factor and 3-level Box-Behnken response surface test for process optimization. Results The optimum extraction conditions were as follows: ultrasonic temperature 71 ℃, ultrasonic time 35 min, ratio of liquid to material 42:1 (V:m), ethanol concentration 57%. Under this condition, the content of polyphenols was (2.33±0.01) mg/g, which was close to the predicted value of (2.33±0.01) mg/g. The predicted value of the model was basically consistent with the actual value. Conclusion Ultrasonic assisted extraction method can effectively extract polyphenols from Rubus bambusarum Focke.