索氏法提取柚籽中柠檬苦素工艺优化研究

Optimization of extraction of limonin from Grapefruit seeds by soxhlet method

中文关键词:  安江香柚  柚籽  柠檬苦素  索氏提取  响应面优化

英文关键词:Anjiang fragrant pomelo  Grapefruit seeds  limonin  Soxhlet extraction  response surface optimization.

中文摘要:

      目的 通过响应面优化得到索氏法提取柚籽中柠檬苦素的最佳工艺。方法 样品粉碎过60目筛, 在一定体积的乙醇水溶液中索氏提取一定时间, 高速冷冻离心机离心得到提取液, 稀释、加样、显色, 在491 nm下测定吸光度, 计算安江香柚籽中柠檬苦素的含量。结果 乙醇浓度、提取时间、液料比对安江香柚籽中柠檬苦素的提取均有显著影响; 响应面优化后的提取工艺为: 乙醇浓度90%、提取时间2.2 h、液料比40:1 (mL/g), 得到柠檬苦素提取率最大值11.36 mg/g。结论 利用索氏法提取安江香柚籽中的柠檬苦素方法可行, 效果明显。

英文摘要:

      Objective To optimize process for extracting limonin from Grapefruit seeds by Soxhlet method by response surface optimization. Methods The sample was pulverized through a 60 mesh sieve, and the mixture is soxed in a certain volume of aqueous ethanol solution for a certain period of time, and the extract is obtained by centrifugation in a high speed refrigerating centrifuge. After dilution, sample loading and color development, the absorbance was measured at 491 nm, and the content of limonin in Anjiangxiang pomelo seeds was calculated. Results Ethanol concentration, extraction time and liquid-to-liquid ratio have significant effects on the extraction of limonin from Anjiangxiang pomelo seeds. The extraction process after optimization of response surface was: ethanol concentration 90%, extraction time 2.2 h, liquid to material ratio 40:1 mL/g, the maximum extraction rate of limonin was 11.36 mg/g. Conclusion The method of extracting limonin from Anjiangxiang pomelo seeds by Soxhlet method is feasible and effective.