海娇颜片中原花青素含量快速测定方法优化

Optimization of rapid content determination method of procyanidin in Haijiaoyan tablets

中文关键词:  原花青素  海娇颜片  铁盐催化比色法  大孔树脂  乙醇沉淀-离心法

英文关键词:procyanidin  Haijiaoyan tablets  colorimetry with ferric ions as catalysis agent  macroporous resin  ethanol precipitation-centrifugation

中文摘要:

      目的 优化快速测定海娇颜片中原花青素含量的分析方法。方法 通过加标回收率法发现采用铁盐催化比色法测定海娇颜片中原花青素时的干扰成分, 并分别采用大孔树脂柱及乙醇沉淀-离心2种方法对样品进行前净化处理。结果 发现海娇颜片中鱼胶原蛋白肽对原花青素的测定存在较大干扰, 优化前测得原花青素含量为1.22%, 经大孔树脂柱净化后测得其含量为2.15%, 而经乙醇沉淀-离心净化后测定其含量为3.11%, 接近理论值。结论 乙醇沉淀-离心法能显著去除干扰成分, 提高原花青素含量测定的准确度, 是较优的样品预处理方法。该研究可为相关产品中原花青素含量快速测定方案的制定提供参考依据。

英文摘要:

      Objective To optimize the method for rapid determination of procyanidin content in Haijiaoyan tablets. Methods The method of spiked recovery was firstly applied to discover the components which had interference on the determination of procyanidin in Haijiaoyan tablets by the typical method of ferric ions as catalyst, and two process methods including macroporous resin column and ethanol precipitation-centrifugation were then applied to pretreat the sample. Results Fish collagen peptide in Haijiaoyan tablets had obvious interference on the determination of procyanidin. The detected content of procyanidin was 1.22% before optimization, 2.15% after purification through macroporous resin column, and 3.11% after the treatment by ethanol precipitation-centrifugation, respectively. And the last one was much closer to the theoretical value. Conclusion Ethanol precipitation-centrifugation method could significantly remove the interfering components and improve the accuracy of determination for procyanidin, which is the better strategy for sample pretreatment. This study could provide reference for the protocol establishment of rapid determination of procyanidin in health products.