高效液相色谱法同时测定抗疲劳功能食品中4种功效成分的研究

Determination of 4 functional compositions in antifatigue health foods by high performance liquid chromatography

中文关键词:  抗疲劳类功能食品  高效液相色谱法  红景天苷  淫羊藿苷  人参皂苷Rg1  葛根素

英文关键词:anti-fatigue functional food  high-performance liquid chromatography  salidroside  icariin  ginsenoside Rg1  puerarin

中文摘要:

      目的 建立高效液相色谱法同时检测抗疲劳类功能食品中红景天苷、淫羊藿苷、人参皂苷Rg1和葛根素4种有效成分的分析方法。方法 样品中的待测组分经乙腈水溶液超声提取、过滤后, 采用SHIFEIDO C18柱(4.6 mm×250 mm, 5 μm)为分离柱, 以乙腈(A)-水(B)为流动相, 梯度洗脱流速为1.0 mL/min; 检测波长为 203 nm; 柱温为40 ℃, 标准曲线法定量。结果 4种化合物分离度良好, 线性相关系数均大于0.9996, 回收率为96.4%~103.7%, 检出限为5.2~50.0 μg/g, 定量限为15.5~151.6 μg/g。结论 本方法简便、准确, 可用于抗疲劳类功能食品中功效成分的检测。

英文摘要:

      Objective To establish a method for the determination of salidroside, icariin, ginsenoside Rg1 and puerarin in anti-fatigue functional foods by high performance liquid chromatography. Methods The target chemicals in samples were ultrasonically extracted with acetonitrile aqueous solution and filtered. The separation was performed on a SHIFEIDO C18 column (4.6 mm×250 mm, 5 μm) and acetonitrile (A)-water (B) was used as mobile phase using gradient elution. The flow rate was 1 mL/min, and the detection wavelength was 203 nm. Column temperature was 40 ℃, and samples were quantified by standard curve method. Results The resolution of the 4 compounds was good, the linear correlation coefficients were all greater than 0.9996, the recovery was 96.4%?103.7%, the limit of detection was 5.2?50.0 μg/g, and the limit of quantification was 15.5?151.6 μg/g. Conclusion This method is simple and accurate, which is suitable for the detection of functional components in anti-fatigue functional foods.