对比福林酚法和高效液相色谱法测定酒神菊属蜂胶和国产蜂胶中酚酸类化合物含量

Comparison of determination of phenolic compounds in the Baccharis dracunculifolia propolis and domestic propolis by Folin-phenol method and high performance liquid chromatography

中文关键词:  酒神菊属蜂胶  国产蜂胶  福林酚法  高效液相色谱法  酚酸类化合物

英文关键词:Baccharis dracunculifolia propolis  domestic propolis  Folin-phenol method  high performance liquid chromatography method  phenolic compounds

中文摘要:

      目的 比较福林酚法和高效液相色谱法测定酒神菊属蜂胶和国产蜂胶中的酚酸含量的差异。 方法 采用福林酚法(Folin-酚法)测总酚酸, 以没食子酸为对照品, 765 nm下检测样品的吸光值; 采用高效液相色谱法(high performance liquid chromatography, HPLC)测总酚酸, 以绿原酸、咖啡酸、异绿原酸A、异绿原酸C、咖啡酸苯乙酯、阿替匹林C为对照品, 优化HPLC洗脱条件, 利用标准品的进样量和色谱峰面积进行线性拟合, 线性回归方程以计算各个单体的含量。结果 Folin-酚法下, 酒神菊属蜂胶总酚酸含量为5.71%~14.68%, 平均为11.34% (n=14), 国产蜂胶总酚酸含量为5.87%~28.99%, 平均值为16.98% (n=12); HPLC法下, 酒神菊属蜂胶总酚酸含量为2.13%~9.20%, 均值为5.71% (n=14), 国产蜂胶总酚酸含量为1.69%~9.13%, 均值为5.05% (n=12)。结论 2种蜂胶都含有比较高含量的酚酸类成分, 不同测定方法下酚酸类成分含量存在明显差异, 选择合适的方法用于检测蜂胶中的酚酸类成分含量显得尤为重要。

英文摘要:

      Objective To compare the determination of phenolic compounds in Baccharis dracunculifolia propolis and domestic propolis by Folin-phenol method and high performance liquid chromatography. Methods The total phenolic acid was determined by Folin-phenol method. The gallic acid was used as the reference substance, and the absorbance of the propolis samples was detected at 765 nm. Total phenolic acid was determined by high performance liquid chromatography, with chlorogenic acid and caffeic acid. Isochlorogenic acid A, isochlorogenic acid C, phenethyl caffeate, and ethipirin C as reference materials. Meanwhile, the HPLC elution conditions were optimized, and the linear fitting was established with standard injection volume and chromatographic peak area. And then, the regression equation was used to calculate the content of each monomer of phenolic acid. Results Under the Folin-phenol method, the total phenolic acid content of the Baccharis dracunculifolia propolis was 5.71%?14.68%, with an average of 11.34% (n=14). The total phenolic acid content of the domestic propolis was 5.87%?28.99%, with an average of 16.98% (n=12). Under the HPLC method, the total phenolic acid content of the Baccharis dracunculifolia propolis was 2.13%?9.20%, the average value was 5.71% (n=14), and the total phenolic acid content of the domestic propolis was 1.69%?9.13%, with the mean was 5.05% (n=12). Conclusion Both propolis contain relatively high content of phenolic acid components, and there are significant differences in the content of phenolic acid components under different determination methods. Therefore, it is particularly important to choose an appropriate method to detect the content of phenolic acid components in propolis.