竞争性酶联免疫法快速检测高产奶牛精料补充料中玉米赤霉烯酮

Rapid detection of zearalenone in high-yielding dairy concentrate supplements by competitive enzyme-linked immunosorbent assay

中文关键词:  酶联免疫吸附法  回收率  玉米赤霉烯酮  饲料

英文关键词:enzyme-linked immunosorbent method  recovery  zearalenone  feed

中文摘要:

      目的 建立竞争性酶联免疫方法检测饲料中玉米赤霉烯酮的分析方法。方法 样品经过60%的甲醇溶液提取, 提取液经过离心后, 取25 ?L提取液, 加入475 ?L稀释缓冲液混合后即为试样液, 取50 ?L试样液采用酶联免疫吸附方法进行检测。在450 nm波长处检测吸光度值。结果 本方法在3 h内完成了饲料中玉米赤霉烯酮含量的测定。在加标浓度为100、200和500 ?g/kg的加标水平下, 玉米赤霉烯酮酶在饲料中的加标回收率为99.5%~122.1%, 变异系数为1.4%~3.0%。结论 该方法快速、准确、灵敏, 适用于饲料中玉米赤霉烯酮的快速筛查检测。

英文摘要:

      Objective To establish a method for rapid detection of zearalenone in high-yielding dairy concentrate supplements by competitive enzyme-linked immunosorbent assay. Methods The sample was extracted with 60% methanol solution. After the extract was centrifuged, 25 μL extraction solution was added, and 475 μL dilution buffer was added to mix the sample solution. The 50 μL sample solution was taken for detection by enzyme-linked immunosorbent assay. Absorbance was detected at 450 nm. Results This method completed the determination of zearalenone in feed within 3 h. At spiked levels of 100, 200, and 500 μg/kg, the spiked recoveries of zearalenone in feed were 99.5% to 122.1%, and the coefficient of variation was 1.4% to 3.0%. Conclusion This method is rapid, accurate and sensitive, which is suitable for rapid screening and detection of zearalenone in feed.