高效液相色谱法测定强化食品中维生素B6的含量

Determination of vitamin B6 in fortified food by high performance liquid chromatography

中文关键词:  高效液相色谱法  维生素B6  吡哆醛  吡哆醇  吡哆胺  强化食品

英文关键词:high performance liquid chromatography  vitamin B6  pyridoxal  pyridoxine  pyridoxamine  fortified food

中文摘要:

      目的 建立了一种高效液相色谱法检测强化食品(婴幼儿配方食品、婴幼儿谷类辅助食品和功能饮料)中维生素B6方法。方法 利用Eclipse Plus C18(5 μm, 4.6 mm×250 mm)色谱柱, 流速1 mL/min, 流动相: 甲醇-(0.2 g/L辛烷磺酸钠, 体积分数为0.25%的三乙胺溶液, 利用乙酸调节pH=3.2±0.1)(V:V=21:79), 进样量 10 μL, 柱温30 ℃实现了对吡哆醛、吡哆醇和吡哆胺3种组分的分离; 在激发波长293 nm, 发射波长395 nm的条件下实现了对这3种组分的检测。结果 吡哆醛、吡哆醇和吡哆胺在0.1~1.0 μg/mL范围内均表现出良好的线性关系, 3种组分的相关系数均大于0.999, 检出限为0.01~0.02 mg/100 g, 定量限为0.03~0.05 mg/100 g, 均满足现行国标要求。3种维生素B6在添加水平为0.05、0.20和0.60 mg/100 g时的加标回收率为89.6%~107.1%, 相对标准偏差(n=6)为1.3%~7.0%。结论 该方法操作简便, 准确度和精密度高, 能够用于对多种常见强化食品中3种维生素B6的检测

英文摘要:

      Objective To establish a method for the determination of vitamin B6 in fortified food by high performance liquid chromatography (HPLC). Methods The effective separation of pyridoxal, pyridoxine and pyridoxamine was achieved with Agilent Eclipse Plus C18 column(5 μm, 4.6 mm×250 mm) by using methanol-0.2 g/L sodium salt and 0.25% trimethylamine (pH=3.2±0.1 adjusting with acetic acid)(V:V=21:79) as the mobile phase . Injection volume is 10 μL and column temperature is 30 ℃. Under the conditions of 293 nm excitation wavelength and 395 nm emission wavelength, the detection of these three components is realized. Results In this method, pyridoxal, pyridoxine and pyridoxamine exhibited excellent linearity over the range of 0.1?1.0 μg/mL, with correlation coefficients of the 3 components all above 0.999. The limits of detection of all the 3 components ranged from 0.01 to 0.02 mg/100 g and the limits of quantitation of them were from 0.03 to 0.05 mg/100 g which met the requirements of the national standard. The average recoveries for three pigments from 3 kinds of common fortified food were in the range of 89.6%?107.1% with relative standard deviations (n=6) of 1.3%?7.0%. Conclusion The method is convenient, accurate and suitable for determination of 3 kinds of Vitamin B6 in common fortified food.