分光光度法测定肉制品中亚硝酸盐含量的不确定度评定

Evaluation of uncertainty in determination of the nitrite content in meat products by spectrophotometry

中文关键词:  分光光度法  亚硝酸盐  肉制品  不确定度

英文关键词:spectrophotometry  nitrite  meat products  uncertainty

中文摘要:

      目的 对分光光度法测定肉制品中亚硝酸盐含量的不确定度进行评定。方法 通过建立不确定度评定数学模型, 分析并计算各不确定度分量, 从而得出合成不确定度和扩展不确定度。结果 肉制品中亚硝酸盐含量6.01 mg/kg, 扩展不确定度为0.25 mg/kg (95%, k=2)。结果表明, 用分光光度法测定肉制品中亚硝酸盐时, 其测量不确定度主要来源于标准曲线制备过程, 特别是标准曲线系列显色定容过程, 而样品称量和样液样液定容引入的不确定度可以忽略不计。结论 该评定方法可为分光光度法测定肉制品中亚硝酸盐含量的测量不确定度评定提供参考。

英文摘要:

      Objective To evaluate the uncertainty of the determination of the nitrite content in meat products by spectrophotometry. Methods Through the establishment of the uncertainty evaluation mathematical model, the main components of uncertainty were analyzed and calculated, and the composite uncertainty and extended uncertainty were obtained. Results When the nitrite content in meat sample was 6.01 mg/kg, its expansion uncertainty was 0.25 mg/kg (95%, k=2). The results showed that the uncertainty of determination of nitrite in meat products by spectrophotometry was mainly due to the process of preparing standard curves, especially the process of color liquid setting capacity of standard curves. The uncertainty caused by sample weighing and solution setting capacity is not important. Conclusion This study can be used as a reference for the uncertainty evaluation for the determination of nitrite content in meat products by spectrophotometry.