自动菌落计数仪在乳品检测中的应用研究

Research on the application of automatic colony counting instrument in dairy products detection

中文关键词:  自动菌落计数仪  快速简便  乳品

英文关键词:automatic colony counter  fast and simple  dairy products

中文摘要:

      目的 对自动菌落计数仪的技术参数、准确度、精密度、重现性等进行试验, 研究其是否满足乳品中菌落总数的计数要求。方法 选用一次性平皿的倾注法和3M测试片法的菌落结果进行计数, 检测项目包括菌落总数、大肠菌群、霉菌酵母、嗜冷菌、乳酸菌等。检测结果分别用仪器法和目测法进行计数, 并对结果进行比较。结果 目测法和仪器法计数结果进行配对T检验分析可知在95% 置信区间P值大于0.05, 在统计学上无显著性差异, 但检测结果偏差10%的符合率达不到100%。重复性偏差为0.43%~5.52%, 重现性试验相对标准偏差为3.60%, 国标方法的标准偏差为3.05%。结论 样品的背景颜色和颗粒度对仪器的计数结果有影响, 可根据实际情况选择使用。

英文摘要:

      Objective To test the technical parameters, accuracy, precision, reproducibility, and so on of the automatic colony counter, and study whether it meets the counting requirements of the total number of colonies in the dairy. Methods The colony results of the disposable plate and the 3M test piece method were used for counting, and the detection items included the total number of colonies, Coliform bacteria, mold yeast, psychrophilic bacteria, Lactobacillu, etcs. The test results were counted by instrumental method and visual method, and the results were compared. Results The paired T test analysis of the visual and instrumental results showed that the P value was greater than 0.05 in the 95% confidence interval, and there was no statistically significant difference in statistics, but the coincidence rate of the test result deviation of 10% was less than 100%. The repeatability deviation was 0.43%?5.52%, the relative standard deviation of the reproducibility test was 3.60%, and the standard deviation of the national standard method was 3.05%. Conclusion The background color and particle size of the sample have an effect on the counting result of the instrument. The automatic colony counter can be selected according to the actual situation.