保健食品中病原微生物的鉴定和溯源分析

Identification and traceability analysis of pathogenic microorganism isolated from healthy food

中文关键词:  保健食品  病原微生物  鉴定  溯源

英文关键词:health food  pathogenic microorganism  identification  traceability

中文摘要:

      目的 对抽检的保健食品病原微生物进行鉴定与溯源分析。方法 采用显微观察、生化反应、VITEK 2 Compact鉴定系统、16S rRNA基因同源性分型, 对从样品和实验环境中分离得到的11株菌株进行鉴定和同源性分析。结果 由抽检的保健食品中分离的细菌分别被鉴定为恶臭假单胞菌、杀鲑气单胞菌、成团泛菌、奇异变形菌、溶血不动杆菌、弗氏柠檬酸杆菌、浅绿气球菌、洋葱伯克霍尔德氏菌、粘质沙雷菌, 其中8株为革兰氏阴性菌, 1株为革兰氏阳性菌; 而由实验环境中分离的2株细菌全部为革兰氏阳性菌, 分别是藤黄微球菌和表皮葡萄球菌; 由16S rRNA基因同源性分析可知, 从样品中分离得到的8株细菌和实验环境中分离得到的2株细菌并不在一个系统发育分支, 亲缘关系较远, 不存在相关性。结论 保健食品中分离的细菌和实验环境中分离的细菌亲缘关系较远, 不存在相关性, 保健食品的污染源与微生物限量检查的实验环境无关, 样品的污染可能是在加工过程中引入的。

英文摘要:

      Objective To identify and trace the pathogenic microorganism of health food. Methods Eleven target-bacteria, isolated from healthy food samples and environment, were identified and traced during homology analysis by microscopic observation, biochemical reaction, VITEK 2 Compact system, 16S rRNA gene homology typing. Results The strains from healthy food samples were appraised as Pseudomonas putida, Aeromonas salmonicida, Pantoea agglomerans, Proteus mirabidis, Acinetobacter haemolyticus, Citrobacter freundii, Aerococcus viridians, Burkholderia cepacia, Serratia marcescens, and 8 strains among them were gram negative, and 1 strain was gram positive; 2 strains form environment were appraised as Micrococcus luteus, Staphylococcus epidermidis and all of them were gram positive. Eight strains from healthy food samples and 2 strain form environment were traced into different types by the analysis of phylogenetic relationships. It seemed that the source of healthy food contamination would be introduced from process and transportation steps, and it was not related to the inspection environment. Conclusion The strains from healthy food samples and the strain form environment are traced into different types and there are not related to the inspection environment. The source of healthy food contamination may be from process and transportation steps.