多功能净化柱净化-超高液相色谱串联质谱法检测云南所售部分面条及面条制品中的6种真菌毒素

Determination of 6 fungal toxins in some noodles and noodle products in Yunnan province by multifunctional purification column purification-ultra performance liquid chromatography tandem mass spectrometry

中文关键词:  面条及其制品  真菌毒素  超高液相色谱-串联质谱法

英文关键词:noodles and their products  mycotoxins  ultra performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立多功能净化柱净化-超高液相色谱-串联质谱法检测云南所售部分面条及面条制品中脱氧雪腐镰刀菌烯醇、3-乙酰脱氧雪腐镰刀菌烯醇、15-乙酰脱氧雪腐镰刀菌烯醇、雪腐镰刀菌烯醇、赭曲霉毒素A和玉米赤霉烯酮6种真菌毒素的含量。方法 样品经乙腈-水-甲酸(70: 29: 1, V:V:V)溶液浸泡提取, 通过Pribo Fast?M226多功能净化柱净化, 采用超高液相色谱-串联质谱的多反应监测模式进行分析, 外标法进行定量。结果 6种真菌毒素在0.05~400 μg/L范围内线性关系良好(r>0.994), 加标回收率为87.6%~101.3%, 相对标准偏差为1.09%~3.65%。81份面条样品中, 脱氧雪腐镰刀菌烯醇检出含量为15.2~664 μg/kg, 检出率为98.77%, 检测值均未超过国家食品安全标准规定的参考限量值, 其余5种真菌毒素均为未检出。结论 本检测方法具有快速便捷、准确性好、灵敏度高、特异性强等特点, 适用于面条中6种真菌毒素的同时测定。

英文摘要:

      Objective To establish a method for determination of deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, fusarium oxysporum, ochratoxin A and zearalenone in some noodles and noodle products sold in Yunnan province by multifunctional purification column purification-ultra performance liquid chromatography tandem mass spectrometry. Methods The sample was extracted by soaking in acetonitrile formic acid water (70:29:1, V:V:V), purified by Pribo Fast?M226 multi-purification column, and analyzed by ultra-high liquid chromatography-tandem mass spectrometry with multiple reaction monitoring mode, and quantificatively analyzed by external standard method. Results The 6 fungal toxins had good linear relationships in the range of 0.05~400 μg/L(r>0.994). The recoveries were 87.6%?101.3%, with the relative standard deviations of 1.09%?3.65%. Among the 81 noodle samples, the detection content of deoxynivalenol was 15.2~664 μg/kg, and the detection rate was 98.77%. The detection values did not exceed the reference limit value specified by the national food safety standards, and the other 5 fungi toxins were not detected. Conclusion This method has the characteristics of fast, convenient, accurate, high sensitivity and strong specificity. It is suitable for simultaneous determination of 6 kinds of fungal toxins in noodles.