高效液相色谱法测定保健食品中抗坏血酸同分异构体含量

Determination of ascorbate acid isomers in health foods by high performance liquid chromatography

中文关键词:  保健食品  抗坏血酸  高效液相色谱法

英文关键词:health food  ascorbic acid  high performance liquid chromatography

中文摘要:

      目的 建立一种同时分离和测定保健食品中抗坏血酸同分异构体含量的高效液相色谱法。方法 样品经20 g/L的偏磷酸溶液超声提取, 选用资生堂CAPCELL PAK C18 ADME亲水色谱柱(4.6 mm×250 mm, 5 μm)分离, 以2.3 g/L磷酸二氢铵水溶液(pH值2.0±0.1)为流动相, 等度洗脱, 流速1.0 mL/min, 检测波长 245 nm。结果 该方法能很好的分离L(+)-抗坏血酸和D(-)-抗坏血酸, 2种抗坏血酸在1.0~50.0 μg/mL范围呈现良好的线性关系, 加标回收率分别为99.69%和100.34%, 检出限均为0.05 μg/mL。结论 该方法简单、快速、准确, 适用于保健食品中抗坏血酸同分异构体含量的测定。

英文摘要:

      Objective To establish a method for simultaneous separation and determination of ascorbate acid isomers in health food by high performance liquid chromatography (HPLC). Methods The sample was ultrasonically extracted with 20 g/L metaphosphoric acid solution, and separated by Shiseido CAPCELL PAK C18 ADME hydrophilic column (4.6 mm × 250 mm, 5 μm) at the flow rate of 1.0 mL/min by isocratic elution using 2.3 g/L ammonium dihydrogen phosphate aqueous solution (pH 2.0±0.1) as mobile phase. The detection wavelength was 245 nm. Results The method could separate L(+)-ascorbic acid and D(-)-ascorbic acid well. The 2 ascorbic acids showed a good linear relationship in the range of 1.0~50.0 μg/mL. The recoveries were 99.69% and 100.34%, respectively. The detection limit was 0.05 μg/mL. Conclusion The method is simple, rapid, accurate and suitable for the determination of ascorbic acid isomers in health food.