气相色谱-质谱法同时测定三七须根中的5种杀菌剂残留

Determination of 5 fungicides residues in fibrous root of Panax notoginseng by gas chromatography-mass spectrometry

中文关键词:  三七须根  杀菌剂残留  QuEChERS  气相色谱-质谱联用法

英文关键词:fibrous root of Panax notoginseng  fungicide residue  QuEChERS  gas chromatography mass spectrometry

中文摘要:

      目的 建立一种气相色谱串联质谱法同时检测三七须根中5种杀菌剂残留量的方法。方法 用乙腈提取三七须根样品中的5种杀菌剂, 经氯化钠盐析, QuEChERS净化后, 利用气相色谱-质谱联法进行定性, 定量检测。结果 5种杀菌剂在0.05~5.00 μg/mL的浓度范围线性关系良好, 相关系数为0.9993~0.9998, 检出限为0.0001~0.0011 mg/kg, 高中低3种加标浓度的平均回收率为84.0%~102.5%, 相对标准偏差在2.52%~5.22% (n=5)。结论 该方法简单灵敏、高效可靠, 适用于三七根须中5种杀菌剂的快速检测。

英文摘要:

      Objective To establish a method for simultaneous determination of five fungicides in fibrous root of Panax notoginseng by gas chromatography-tandem mass spectrometry (GC-MS). Methods Totally 5 kinds of fungicides in the samples of fibrous root of Panax notoginseng were extracted with acetonitrile, salted out by sodium chloride, purified by QuEChERS, then qualitative and quantitative detected by gas chromatography-mass spectrometry. Results All 5 fungicides had good linear relationships in the range of 0.05?5.00 μg/mL, and the correlation coefficients were 0.9983?0.9998. The limits of detection were 0.0001?0.0011 mg/kg. The average recoveries at 3 spiked levels (high, middle and low) were 84.0%?102.5%, with the relative standard deviations of 2.52%?5.22% (n=5). Conclusion The method is simple, sensitive, efficient and reliable, and is suitable for the rapid detection of 5 fungicides in Panax notoginseng root.