云南文山三七须根急性毒性和诱变毒性作用的研究

Study on acute toxicity and mutagenic toxicity of Panax notoginseng fibrous root in Wenshan, Yunnan

中文关键词:  云南文山三七须根  急性毒性  Ames试验  微核  精子畸形

英文关键词:fibrous root of Panax notoginseng in Wenshan, Yunnan  acute toxicity  Ames test  micronucleus  sperm deformity

中文摘要:

      目的 探讨云南文山三七须根急性毒性和诱变毒性作用。方法 急性毒性采用一次限量法, 剂量设为10000 mg/kg BW。Ames实验使用的菌株为鼠伤寒沙门氏菌组氨酸缺陷型菌株TA97、TA98、TA100、TA102。实验分别设每皿5000、1000、200、40、8 μg剂量组, 同时设阴性、阳性对照组, 采用平板掺入法, 观察每皿回变菌落数。微核及精子畸形试验均使用SPF级昆明种小鼠, 实验设1250、2500、5000 mg/kg BW 3个剂量组、一个阴性对照组及一个阳性对照组。微核试验采用30 h给受试物法, 检测骨髓嗜多染红细胞微核率。精子畸形试验于首次染毒后第35 d处死动物, 观察精子畸形率。结果 云南文山三七须根对受试动物的急性毒性(LD50)>10000 mg/kg BW。与阴性对照组比较, 云南文山三七须根各剂量组在鼠伤寒沙门氏菌试验(Ames试验)中实验结果为阴性, 骨髓细胞微核试验表明未引起微核发生率增加, 对小鼠精子畸变无明显影响(P＞0.05)。结论 云南文山三七须根对昆明种小鼠的急性经口毒性属实际无毒, 在Ames实验中未呈现致突变作用, 未见诱导小鼠骨髓嗜多染红细胞微核形成的能力, 未见有致小鼠精子畸形作用。

英文摘要:

      Objective To investigate the acute toxicity and mutagenic toxicity of Panax notoginseng fibrous root in Wenshan, Yunnan. Methods Acute toxicity was determined by one-time limit method at a dose of 10000 mg/kg BW. The strain used in the Ames test was Salmonella typhimurium histidine-deficient strains TA97, TA98, TA100 and TA102. In the experiment, the dose groups of 5000, 1000, 200, 40 and 8 microns per plate were set respectively, and the negative and positive control groups were set at the same time. The number of bacterial colonies in each plate was observed by plate incorporation method. Micronucleus and sperm malformation tests were conducted in SPF grade kunming mice, with 3 dose groups of 1250, 2500 and 5000 mg/kg BW, one negative control group and one positive control group. The micronucleus test was carried out for 30 h to test the micronucleus rate of bone marrow polychromatic erythrocytes. The sperm deformity test was performed on the 35th day after the first exposure, and the sperm deformity rate was observed. Results The acute toxicity (LD50) of fibrous root of Panax notoginseng in Wenshan, Yunnan province was more than 10000 mg/kg BW. Compared with the negative control group, the results of Salmonella typhimurium test (Ames test) in each dose group of Panax notoginseng fibrous root in Wenshan, Yunnan were negative. The micronucleus test of bone marrow cells showed that the incidence of micronucleus did not increase, and there was no significant effect on sperm aberration in mice (P＞0.05). Conclusion The acute oral toxicity of Panax notoginseng fibrous roots in Wenshan, Yunnan province to Kunming mice was actually non-toxic. No mutagenic effect was observed in Ames test, no ability to induce micronucleus formation of polychromatic erythrocyte in bone marrow of mice was observed, and no sperm deformity was observed in mice.