黄曲霉毒素B1的检测方法

Detection method for aflatoxin B1

中文关键词:  黄曲霉毒素B1  薄层层析法  高效液相色谱法  酶联免疫吸附法  金标试纸法

英文关键词:aflatoxin B1  thin–layer chromatography  high performance liquid chromatography  enzyme–linked immunosorbent assay  gold labeled immunochromatographic assay

中文摘要:

      随着农业市场的不断发展, 伴随产生了一系列的食品安全质量问题, 尤其是毒性最强的黄曲霉毒素B1所引起的安全问题。粮食安全问题是一个不容小觑的重大问题, 现在也越来越受到老百姓的重视。本文介绍了黄曲霉毒素B1(aflatoxin B1, AFB1)及其毒性危害; 综述了几种黄曲霉毒素B1的检测方法, 分别包括: 薄层层析法(thin–layer chromatography, TLC)、高效液相色谱法(high performance liquid chromatography, HPLC)、酶联免疫吸附法(enzyme linked immunosorbent assay, ELISA)、金标试纸法(gold labeled immunochromatographic assay, GICA)等, 并对这几种方法进行了分析和比较, 以期为黄曲霉毒素B1的监测提供参考。

英文摘要:

      A series of food safety and quality problems have arisen with the continuous development of the agricultural market, especially the safety problems caused by the most toxic aflatoxin B1. Food security is a major issue that should not be underestimated, and now people are paying more and more attention to it. This paper introduced aflatoxin B1, its toxicity harm and detection methods, analyzed and compared the methods for the detection of aflatoxins B1, including thin–layer chromatography (TLC), high performance liquid (HPLC),enzyme linked immunosorbent assay (ELISA) and gold labeled immunochromatographic assay (GICA), in order to provide reference for the monitoring of aflatoxin B1.