高效液相色谱法鉴别蜂蜜中掺入蜂王浆来源的蛋白质

Identification of proteins from honey adulterated with royal jelly by high performance liquid chromatography

中文关键词:  蜂蜜掺假  蛋白质  液相色谱

英文关键词:adulteration of honey  protein  liquid chromatography

中文摘要:

      目的 建立高效液相色谱法鉴别蜂蜜中掺入蜂王浆来源的蛋白质。方法 样品经前处理后, 经Diamonsil Plus C18 色谱柱(150 mm×4.6 mm, 5 μm)分离, 以乙腈和0.1%磷酸水溶液为流动相进行等度洗脱, 外标法定量检测蜂蜜中10-羟基-2-癸烯酸(10-hydroxy-2-decanoic acid, 10-HDA)的含量, 确定是否掺入蜂王浆。 结果 基于161个各产区的纯正蜂蜜数据, 提出纯正蜂蜜中10-羟基-2-癸烯酸含量应小于0.50 mg/kg。5种蜂蜜样本的回收率为96.9%~101.6%, 相对标准偏差为1.6%~5.9%。对121个日常检测样品进行鉴定, 其中16个有10-HAD超过判定值, 被认为掺有蜂王浆来源的蛋白质。结论 该方法准确可靠, 适用于蜂蜜中10-羟基-2-癸烯酸含量的测定以及蜂蜜中掺入蜂王浆来源蛋白质的鉴别。

英文摘要:

      Objective To establish a method for the identification of proteins from honey adulterated with royal jelly by high performance liquid chromatography (HPLC). Methods After pretreatment, the samples were separated on a Diamonsil Plus C18 column (150 mm × 4.6 mm, 5 μm) at the flow rate of 0.20 mL/min by isocratic elution using acetonitrile and 0.1% phosphoric acid in aqueous solution as mobile phase. The content of 10-hydroxy-2-decanoic acid (10-HDA) in honey was quantitatively determined by external standard method to determine whether royal jelly was added. Results Based on the data of pure honey from 161 producing areas, it was suggested that the content of 10-hydroxy-2-decenoic acid in pure honey should be less than 0.50 mg/kg. The recoveries of the 4 honey samples were 97.7%?101.6%, and the relative standard deviations were 1.6%?5.9%. Identification of 121 daily testing samples, 16 of which have 10-HAD exceeding the judgment value, were considered to be spiked with royal jelly-derived protein. Conclusion This method is accurate and reliable, and is suitable for the determination of 10-hydroxy-2-decenoic acid in honey and the identification of royal jelly-derived proteins in honey.