异硫氰酸烯丙酯酶解转化工艺的优化

Optimization of enzymatic hydrolysis of allyl isothiocyanate

中文关键词:  黑芥子酶  酶解转化  烯丙基异硫氰酸酯  正交试验

英文关键词:myrosinase  enzymatic hydrolysis  allyl isothiocyanate  orthogonal test

中文摘要:

      目的 优化酶解黑芥子苷转化成异硫氰酸烯丙酯(allyl isothiocyanate, AITC)的水解条件。方法 在已知AITC的测定条件下, 以AITC转化率为考察指标, 对温度、pH、Zn2+离子添加量、乙二胺四乙酸(ethylene diamine tetraacetic acid, EDTA)添加量及酶来源进行单因素试验以及设计五因素四水平的正交试验, 确定最优转化工艺条件。结果 黑芥子苷对AITC最优转化条件为: 黑芥子酶来源为樱桃萝卜种子、pH为7.00、Zn2+的添加量为1.00 mmol/L、转化温度为40 ℃、螯合EDTA添加量为0.30 mmol/L。最优转化条件下AITC的转化率为70.32%。结论 本研究的实验设计简单且能真实的提高AITC的转化率, 对酶解法制备AITC提供数据依靠。

英文摘要:

      Objective To optimize the hydrolysis condition for allyl isothiocyanate (AITC) transformed from myrosinase. Methods The transformation rate of AITC was taken as the index under the condition of known determination of AITC. Single factor experiments (temperature, pH, additive amount of Zn2+, additive amount of EDTA and resource of myrosinase) and 5 factors and 4 levels orthogonal experiments were designed to determine the optimal enzymatic hydrolysis conditions. Results The optimum transformation conditions for enzymatic hydrolysis of myroside to AITC were as follows: myrosinase originated from cherry radish seeds, pH 7.00, Zn2+ 1 mmol/L, temperature 40 ℃, EDTA dosage 0.30 mmol/L. The conversion of AITC was 70.32% under the optimal hydrolysis conditions. Conclusion The experimental design of this study was simple and could actually improve the conversion rate of AITC, which provided data support for the preparation of AITC by enzymatic hydrolysis.