紫外分光光度法测定广之源胶囊中总皂苷含量的优化

Optimization of determination of total saponins in Guangzhiyuan capsules by ultraviolet spectrophotometry

中文关键词:  总皂苷  质量标准  DM-130大孔树脂  紫外-可见光分光光度法

英文关键词:total saponins  quality standards  DM-130 macroporous resin  ultraviolet spectrophotometry

中文摘要:

      目的 建立紫外分光光度法测定广之源胶囊中总皂苷含量的分析方法。方法 样品采用85%乙醇超声提取, 提取液经DM-130大孔树脂柱, 采用水-乙醇溶剂系统对样品的皂苷成分进行洗脱、纯化、富集, 以人参皂苷Re为对照品, 利用紫外可见分光光度法测定其总皂苷含量。结果 该人参皂苷Re在0~191.2 μg范围内的线性回归方程Y=0.0043X?0.0057(R2=0.9995), 相对标准偏差(relative standard deviation, RSD)为1.3%和2.3%, 加样回收率为99.7%。结论 该方法简单、快捷、高效, 适用于广之源胶囊的总皂苷含量测定。

英文摘要:

      Objective To establish a method for the determination of total saponin content in Guanghuaziyuan capsule by ultraviolet spectrophotometry. Methods Samples were extracted with 85% ethanol by ultrasound extraction, and the extract was sampled on a DM-130 macroporous adsorption resin. The saponins were eluted, purified and enriched by the water-ethanol solvent system. Using ginsenoside Re as reference substance, the content of total saponins was determined by ultraviolet spectrophotometry. Results The linear regression equation of ginsenoside Re was Y=0.0043X?0.0057 (r2=0.9995) in the range of 0?191.2 μg. The relative standard deviations were 1.3% and 2.3%, and the recovery of sample addition was 99.7%. Conclusion This method is simple, rapid and efficient, and it is suitable for the determination of total saponins in Guangzhiyuan capsules.