微波消解-电感耦合等离子体质谱法同时测定食用菌中的16种金属元素

Determination of 16 kinds of metal elements in edible fungi by microwave digestion and inductively coupled plasma-mass spectrometry

中文关键词:  食用菌  金属元素  微波消解  电感耦合等离子体发射质谱法

英文关键词:edible fungi  metal elements  microwave digestion  inductively coupled plasma-mass spectrometry

中文摘要:

      目的 建立微波消解-电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry, ICP-MS)同时测定食用菌中16种金属元素含量的分析方法。方法 采用硝酸-双氧水微波消解进行前处理, ICP-MS检测的方法进行测定, 并对方法的线性范围、检出限、精密度和回收率等进行考察, 并将该方法用于5种食用菌样品中16种金属元素含量的及检测。结果 各元素在相应的浓度标准曲线范围内呈良好的线性关系, 相关系数为0.9992~1.0000, 检出限为0.001~0.80 μg/L, 回收率为80.2%~108.3%, 相对标准偏差为0.08%~3.88%。结论 该方法测定食用菌中的重金属元素操作方便, 灵敏度高, 干扰较小, 检出限低, 精密度高, 准确度较好, 能够满足食用菌中多种元素同时检测的需要。研究发现食用菌尤其是木耳类含铝元素相对较高, 同时含有微量元素锌和铜, 摄入需要适当。

英文摘要:

      Objective To establish a method for simultaneous determination of 16 metal elements in edible fungi by microwave digestion and inductively coupled plasma mass spectrometry (ICP-MS). Methods The samples were treated by H2O2 microwave digestion, and 16 metal elements in 5 kinds of edible fungi were analyzed by ICP-MS. The linear range, limit of detection, precision and recovery rate of the method were investigated, and the method was applied to detect 16 kinds of metal elements in 5 kinds of edible fungi samples. Results Each element had a good linear relationship in the range of the corresponding concentration standard curve. The correlation coefficient was 0.9992?1.0000, the limit of detection was 0.001?0.80 μg/L and the recovery rates were 80.2%?108.3%, with the relative standard deviation between 0.08%?3.88%. Conclusion This method is simple, less interference, high sensitivity, low detection limit, high precision and good accuracy in the determination of various elements in edible fungi, and is suitable for simultaneous determination of various elements in edible fungi. In addition, it is found that edible fungi, especially auricularia, contains relatively high aluminum, and trace elements like zinc and copper. It is suggested that people should eat edible fungi properly.