基于高效液相色谱图谱结合化学计量学的红参指纹图谱研究

Research on fingerprint of red ginseng based on high performance liquid chromatography combined with chemometrics methods

中文关键词:  红参  指纹图谱  高效液相色谱法  聚类分析

英文关键词:red ginseng  fingerprint  high performance liquid chromatography  cluster analysis

中文摘要:

      目的 建立红参药材高效液相色谱(high performance liquid chromatography, HPLC)指纹图谱分析方法。方法 采用Diamonsil Plus C18色谱柱(250 mm×4.6 mm, 5 μm), 以乙腈–水为流动相梯度洗脱, 柱温30 ℃, 流速为1.0 mL/min, 检测波长为203 nm。通过相似度评价、聚类分析对指纹图谱进行评价。结果 建立的 21批红参的对照指纹图谱, 确定25个共有峰, 通过对照品对比对其中14个共有峰进行了指认, 21批红参药材, 除S4, S20, 其相似度均大于0.97。结论 该方法操作简便、准确可靠、重复性好, 普适性高, 为红参药材质量控制提供有效手段。

英文摘要:

      Objective To establish high performance liquid chromatography (HPLC) method for chromatographic analysis of red ginseng. Methods Diamonsil Plus C18 column (250 mm×4.6 mm, 5 μm) was used, the gradient was eluted with acetonitrile-water as the mobile phase, the column temperature was 30 ℃, the flow rate was 1.0 mL/min, and the detection wavelength was 203 nm. The fingerprints were evaluated by cluster analysis and principal component analysis. Results The control fingerprints of 21 batches of red ginseng were established, and 25 common peaks were identified. The common peaks of n’g were identified by comparison of the reference materials. The similarity of 21 batches of red ginseng herbs was greater than 0.97. Conclusion This method is simple, accurate, reliable, reproducible and universal, which provides an effective mean for quality control of red ginseng.