酸化处理的聚酰胺粉方法测定巴西绿蜂胶粉中总黄酮

Determination of total flavonoids in Brazilian green propolis powder by acidified polyamide powder

中文关键词:  聚酰胺粉  总黄酮  巴西绿蜂胶粉

英文关键词:polyamide powder  total flavonoids  Brazil green propolis powder

中文摘要:

      目的 建立以酸化聚酰胺粉吸附黄酮测定巴西绿蜂胶粉中总黄酮的方法。方法 将酸化聚酰胺粉吸附总黄酮, 经甲醇洗脱, 用经酸化的标准溶液制备标准曲线, 测定样品中的总黄酮。结果 采用酸化聚酰胺粉吸附黄酮, 总黄酮在2.137~21.370 μg/mL(r=0.99956)范围内具有良好的线性关系, 平均加标回收率为98.9% (RSD=0.7%, n=9)。结论 该方法有效提高总黄酮的洗脱效率, 回收率良好, 能够提升检测结果的准确性。

英文摘要:

      Objective To establish a method for determination of total flavonoids in Brazilian green propolis powder by acidizing polyamide powder washing. Methods In the method, the acidizing polyamide powder is acidified to adsorb the total flavonoids, and then eluted with methanol. Prepare standard curve with acidified standard solution to determine the total flavonoids in the sample. Results The total flavonoid had a good linear relationship in the range of 2.137 to 21.370 μg/mL (r=0.99956), and the average recovery was 98.9% (RSD=0.7%, n=9). Conclusion The method can improve the elution efficiency and recovery rate of total flavonoids, it can improve the accuracy of the test results.