新疆野生樱桃李果皮中花色苷提取工艺优化及成分鉴定

Optimization of extraction technology and identification of anthocyanin in Prunus cerasifera from Xinxiang

中文关键词:  野生樱桃李  花色苷  提取工艺  液相色谱-电喷雾-质谱法  结构鉴定

英文关键词:Prunus cerasifera  anthocyanins  extraction process  liquid chromatography electrospray ionization- mass spectrometry  structural identification

中文摘要:

      目的 优化新疆野生樱桃李果皮中花色苷的提取工艺并鉴定其中主要花色苷成分。方法 采用单因素实验和L9(34)正交试验优化花色苷的提取工艺, 并用色价法、示差法测定其含量。通过液相色谱-电喷雾-质谱(liquid chromatography electrospray ionization-mass spectrometry, LC-ESI-MS)方法初步鉴定提取物中花色苷的主要成分。结果 野生樱桃李果皮花色苷的最优提取条件为: 溶剂1% HCl-甲醇, 固液比1:5, 温度55 ℃, 时间80 min, 紫皮野生樱桃李总花色苷含量480.81 mg/100 g。野生樱桃李果皮主要花色苷为矢车菊-3-半乳糖苷、矢车菊-3-葡萄糖苷、矢车菊-3-芸香糖苷、矢车菊-3-木糖苷。结论 本研究为新疆野生樱桃李果皮中花色苷类化合物的提取和成分鉴定提供重要实验数据。

英文摘要:

      Objective To optimize the extraction technology of anthocyanin in Prunus cerasifera from Xinxiang and identify the main anthocyanin components. Methods The extraction process of anthocyanin was optimized by single factor experiment and L9(34) orthogonal experiment, and the anthocyanin content was determined by color valence method and differential method. Results The optimal extraction conditions of anthocyanin from wild cherry peel were as follows: solvent 1% HCl-methanol, solid-liquid ratio 1:5, temperature 55 ℃, time 80 min, and the total anthocyanin content of Prunus cerasifera from Xinxiang was 480.81 mg/100 g. The main anthocyanins of Prunus cerasifera purple skin were cornflower-3-galactosin, cornflower-3-glucoside, cornflower-3-rutin glucoside, cornflower-3-xylose. Conclusion This study provides important experimental data for extraction process and component identification for anthocyanin from prunus cerasifera.