评定高效液相色谱法测定植物油中苯并芘的不确定度

Evaluate the uncertainty of determination of benzo (a) pyrene in vegetable oil by high performance liquid chromatography

中文关键词:  植物油  苯并芘  不确定度  高效液相色谱法

英文关键词:vegetable oil  benzo (a) pyrene  uncertainty  high performance liquid chromatography

中文摘要:

      目的 评定高效液相色谱法测定植物油中苯并芘含量的不确定度。方法 采用高效液相色谱法测定植物油中苯并芘的含量, 建立数学模型, 根据不确定度评定相关规则和标准对实验过程中的不确定度因素包括标准溶液的配制、标准曲线的拟合、回收率等进行不确定度评定。结果 当植物油中苯并芘含量为1.36 μg/kg时, 其扩展不确定度为0.18 μg/kg (P=95%, k=2)。结论 植物油中苯并芘测定的不确定度主要来源有标准溶液的配制、标准曲线拟合和重复性。

英文摘要:

      Objective To evaluate the uncertainty of the benzo (a) pyrene in vegetable oil by high performance liquid chromatography (HPLC). Methods The content of benzo (a) pyrene in vegetable oil was detected by HPLC. Based on the uncertainty evaluation and standard, a mathematical model was established, and the uncertainty resource of the experience process, including the preparation of standard curve, the fitting of standard curve and the recovery were evaluated. Results When the content of benzo (a) pyrene in vegetable oil was 1.36 μg/kg, the expanded uncertainty was 0.18 μg/kg (P=95%, k=2). Conclusion The uncertainty of determination of Benzo (a) pyrene in vegetable oil mainly comes from the preparation of standard solution, standard curve fitting and repeatability.