基于纳米金粒子的猪源性成分快速比色检测

Rapid colorimetric detection of pork components based on gold nanoparticles

中文关键词:  肉类掺假  纳米金粒子  比色法  猪源性成分

英文关键词:meat adulteration  gold nanoparticles  colorimetry  pork ingredients

中文摘要:

      目的 开发一种基于纳米金粒子快速比色的猪源性成分快速检测方法。方法 选取猪线粒体基因中特异性DNA片段为靶点, 利用互补单链DNA与纳米金粒子偶联为探针, 提取检测目标DNA后在盐溶液中进行检测。结果 探针上DNA结合检测目标后, 纳米金粒子失去单链DNA的保护, 发生聚集, 由红色变为蓝色, 实现裸眼可视检测。核酸偶联的最佳用量为0.1 mmol/L的核酸添加30 μL, 方法的检出限为5%。 结论 这种比色检测可以在不使用复杂仪器的条件下将猪肉与其牛肉类产品区分开来, 方法快捷迅速, 对肉制品掺假的防控具有重要意义。

英文摘要:

      Objective To develop a rapid detection method for pig-derived components based on fast colorimetric determination of gold nanoparticles. Methods A specific DNA fragment of the porcine mitochondrial gene was selected as a target, the complementary single-stranded DNA was coupled with the nano gold particles as a probe, and the target DNA was extracted and detected in a salt solution. Results After DNA binding detection on the probe, the gold nanoparticles lost the protection of single-stranded DNA, and they aggregated from red to blue, enabling visual inspection of naked eyes. The optimal amount of nucleic acid coupling was 30 μL for a 0.1 mmol/L nucleic acid, and the limit of detection of the method was 5%. Conclusion This colorimetric test can be used to distinguish pork from beef products without using complicated instruments, which is of great significance for prevention and control of adulteration of meat products.