超高效液相色谱-串联质谱法测定马铃薯中α-茄碱和α-卡茄碱

Determination of α-solanine and α-chaconine in potato by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  α-茄碱  α-卡茄碱  超高效液相色谱-串联质谱法  马铃薯

英文关键词:α-solanine  α-chaconine  ultra performance liquid chromatography-tandem mass spectrometry  potato

中文摘要:

      目的 建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)测定马铃薯中α-茄碱和α-卡茄碱的含量。方法 样品经80%甲醇水(V:V, 含0.1%甲酸)溶液提取、稀释后在正离子模式下以电喷雾电离串联质谱进行测定, 外标法定量。结果 α-茄碱和α-卡茄碱在50~1000 μg/L范围内线性良好, 相关系数＞0.99, 方法检出限为20 μg/kg, 定量限为50 μg/kg。在3、5、10 mg/kg的添加水平下, α-茄碱和α-卡茄碱平均回收率在83.5%~96.0%之间, 相对标准偏差(relative standard deviation, RSD), (n=6)在2.97%~5.41%之间。结论 本方法快速、准确、灵敏, 可应用于马铃薯中α-茄碱和α-卡茄碱的同时检测。

英文摘要:

      Objective To establish an analytical method for determination of α-solanine and α-chaconine in potato by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The samples were extracted by 80% methanol (V:V, contain 0.1% formic) and determined by multiple reaction monitoring (MRM) mode with electrospray ionization source and were quantified by external standard method. Results All of the analytes showed good linearity in the range of 50?1000 μg/kg and the correlation coefficients (r2) were all above 0.99. The limits of detection (LODs) for α-solanine and α-chaconine were 20 μg/kg and the limits of quantification (LOQs) were 50 μg/kg. The average recoveries for the potato samples at 3 spiked levels (3, 5, 10 mg/kg) ranged from 83.5% to 96.0%, with the relative standard deviations (RSDs, n=6) of 2.97%?5.41%. Conclusion The proposed method is rapid, accurate and sensitive, which can be applied for the simultaneous detection of α-solanine and α-chaconine in potato.