基于暴露限值法与数学模型法的某市居民食用植物油中黄曲霉毒素B1的膳食暴露风险评估

Dietary exposure risk assessment of aflatoxin B1 in edible vegetable oil of residents based on margin of exposure and digital method

中文关键词:  黄曲霉毒素B1  植物油  暴露风险  暴露评估

英文关键词:aflatoxin B1  vegetable oil  risk  exposure assessment

中文摘要:

      目的 运用暴露限值法与数学模型法2种不同的方法, 评估某市居民从食用植物油中摄入黄曲霉毒素B1(aflatoxin B1, AFB1)的膳食风险, 并提出建议。方法 采用分层随机法, 抽取广东省某市生产及流通领域中食用植物油, 分别用酶联免疫吸附测定法(enzyme-linked immunosorbent assay, ELISA)和高效液相色谱-柱后衍生法(high performance liquid chromatography, HPLC)检测不同年份样品的AFB1含量, 结合该市居民膳食消费量计算食用油中AFB1的暴露水平, 运用暴露限值(margin of exposure, MOE)法与数学模型法进行膳食风险评估。结果 共采集的341份食用植物油, AFB1检出率为66.0%; 花生油样品检出率和含量均高于其余品种(P<0.05); 散装样品检出率和含量明显高于定型包装样品(P<0.05)。超市和餐饮单位的样品AFB1含量较其它场所低(P<0.05); 不同地区的样品检出率和含量差异无统计学意义(P>0.05)。该市居民食用油AFB1暴露水平为3.12 ng/kg bw/d, MOE值为80, 对于肝癌发病率的贡献为0.1248/10万人。结论 该市居民食用植物油AFB1膳食暴露水平和致肝癌发病的贡献较低, 但仍应引起足够和持续的公共卫生关注, 尤其对于散装花生油应优先采取风险管理措施, 加大监管力度, 并做好人群健康饮食习惯指导。

英文摘要:

      Objective To evaluate the dietary risk of aflatoxin B1 (AFB1) in food oil from a city's residents using exposure limit method and mathematical model method, and give some advice. Methods Edible vegetable oil in the field of production and circulation in Guangdong province was extracted by stratified random method. The AFB1 content was analyzed by enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC). Based on the dietary consumption of residents in this city, the exposure level of AFB1 in edible oil was calculated, and the dietary risk was assessed by margin of exposure (MOE) method and mathematical model method. Results A total of 341 edible vegetable oils were collected, and the detection rate of AFB1 was 66.0%. The detection rate and content of peanut oil were higher than other varieties (P<0.05). The detection rate and content of bulk samples were significantly higher than that of packaged samples (P<0.05). The content of AFB1 in supermarkets and catering units was lower than that in other places (P<0.05). There was no statistically significant difference in sample detection rate and content in different regions (P>0.05). The city’s residents’ AFB1 exposure level was 3.12 ng/kg bw/d, and the MOE value was 80. The contribution to the incidence of liver cancer was 0.1248/100000. Conclusions The city's residents have a lower contribution to the dietary exposure of AFB1 and the incidence of liver cancer, but it should still cause adequate and sustained public health concerns. In particular, priority should be given to risk management measures for bulk peanut oil, and supervision should be strengthened, and healthy dietary habits of the population should be well guided.