原型胶原蛋白的提取分离及其结构鉴定

Extraction and separation of prototype collagen and its structure identification

中文关键词:  原型胶原蛋白  提取分离  结构鉴定

英文关键词:prototype collagen  extraction and separation  structure identification

中文摘要:

      目的 比较不同产品中的原型胶原蛋白构型和理化性质差异。方法 经酶法、盐析等步骤对市售胶原蛋白产品进行提取分离纯化, 利用氨基酸测序技术、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳( sodium dodecyl sulfate-polyacrylamide gel electrophoresis , SDS-PAGE)和圆二色谱技术对其结构进行鉴定。结果 1号胶原蛋白为变性胶原蛋白, SDS-PAGE方法未检测到蛋白的电泳条带, 表明其无明显的空间结构; 2号胶原蛋白存在混杂现象, 为非纯的原型胶原蛋白; 3号胶原蛋白为典型的Ⅱ型胶原蛋白结构特征, SDS-PAGE结果中存在单一的α链和β链, 与文献资料相符合。结论 3种胶原蛋白均具有典型的胶原蛋白蛋白氨基酸组成, 但由于生产工艺的不同、物种来源、部位的差异, 导致表现出了3种不同的空间构型。

英文摘要:

      Objective To compare the differences in prototype collagen configuration and physicochemical properties in different products. Methods Commercially available collagen products were extracted, separated and purified by enzymatic method, salting out and other steps. Their structures were identified by amino acid sequencing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism. Results Collagen 1 was a denatured collagen, and the electrophoresis band of the protein was not detected by SDS-PAGE, indicating that it had no obvious spatial structure. Collagen 2 had a mixed phenomenon, which was a non-pure prototype collagen. Collagen 3 was a typical type II collagen structural feature. There were single alpha and beta chains in the SDS-PAGE results, which were consistent with the literature. Conclusion All 3 kinds of collagen have typical amino acid composition of collagen protein, but due to different production processes, differences in species source and location, 3 different spatial configurations are exhibited.