微波消解-电感耦合等离子体质谱法测定食用菌基质中4种重金属

Determination of 4 kinds of heavy metals in edible fungi by microwave digestion and inductively coupled plasma-mass spectrometry

中文关键词:  食用菌  微波消解  电感耦合等离子质谱  重金属  检测

英文关键词:edible fungi  microwave digestion  inductively coupled plasma-mass spectrometry  heavy metals  detection

中文摘要:

      目的 建立一种微波消解-电感耦合等离子质谱法(inductively coupled plasma-mass spectrometry, ICP-MS)测定食用菌基质中有害重金属砷(As)、汞(Hg)、铅(Pb)和镉(Cd)含量的方法。方法 采用硝酸和过氧化氢对食用菌基质样品进行微波消解, 消解液经稀释后用电感耦合等离子体质谱仪测定, 在线内标法定量, 采用国家标准物质土壤(GBW07427)和杨树叶(GBW07604)以及重复性试验, 并经过3个水平的加标回收试验进行验证。结果 测定的不同品种食用菌基质中的4种重金属元素的加标回收率在90.7%~105.3%之间, 相对标准偏差在0.88%~4.03%之间。结论 该方法稳定性好、灵敏度高、定量准确, 提高了检测效率, 适用于食用菌基质中As、Hg、Pb和Cd, 4种重金属快速、准确的定量分析。

英文摘要:

      Objective To establish a method for the determination of heavy metals, including plumbum (Pb), cadmium (Cd), mercury (Hg) and arsenic (As) in edible fungi by microwave digestion and inductively coupled plasma-mass spectrometry (ICP-MS). Methods Nitric acid and hydrogen peroxide were used for microwave digestion for edible fungi samples. The digestion solution was diluted and determined by ICP-MS, and quantified by on-line internal standard method. It was verified by national standard material soil (GBW07427) and poplar leaves (GBW07604) and repeatability test and 3 levels of spike recovery test. Results The recoveries of 4 heavy metals in different edible fungus matrix were between 90.7% and 105.3%, and the relative standard deviations (RSDs) were between 0.88% and 4.03%. Conclusion This method has good stability, high sensitivity, accurate quantification and improves detection efficiency. It is suitable for the rapid and accurate quantitative analysis of As, Hg, Pb and CD in edible fungi matrix.