霍山石斛口服液安全性研究

Study on the safety of Dendrobium huoshanense oral solution

中文关键词:  霍山石斛口服液  可食用  安全性评价

英文关键词:Dendrobium huoshanense oral solution  edible  safety evaluation

中文摘要:

      目的 评价霍山石斛口服液食用的安全性。方法 依据GB 15193.1-2014《食品安全国家标准 食品安全性毒理学评价程序》, 对霍山石斛口服液进行急性毒性实验、Ames实验、小鼠骨髓细胞微核实验、小鼠精子畸形实验、30 d喂养实验。结果 小鼠对霍山石斛口服液最大耐受剂量(maximal tolerable dose, MTD)大于40 mL/kg BW, 属无毒级; Ames实验显示小鼠骨髓细胞微核试验和小鼠精子畸形实验均检测为阴性; 当霍山石斛口服液剂量达到10.0 mL/kg BW时, 仍未见实验动物出现中毒体征。结论 霍山石斛口服液无毒副作用, 无遗传毒性, 安全性高, 可长期食用。

英文摘要:

      Objective To evaluate the safety of Dendrobium huoshanense oral solutionfor for consumption. Methods According to GB 15193.1-2014 Food safety national standard-Food safety toxicology evaluation procedure, acute toxicity test, Ames test, mouse bone marrow cell micronucleus test, mouse sperm abnormality test and 30 d feeding experiment were conducted on Dendrobium huoshanense oral solution. Results The maximum tolerable dose (MTD) of the mice to Dendrobium huoshanense oral solution was greater than 40 mL/kg BW, which was non-toxic. Ames experiments showed that mouse bone marrow cells micronucleus test and mouse sperm abnormality test were negative. When the oral dose of Dendrobium huoshanense oral solution reached 10.0 mL/kg BW, no poisoning signs were found in the experimental animals. Conclusion Dendrobium huoshanense oral solution has no toxic side effects, no genotoxicity, high safety and it is suitable for long-term consumption.