液相色谱—电感耦合等离子体质谱法分析大米中的砷形态

Analysis of arsenic morphology in rice by liquid chromatography-inductively coupled plasma mass spectrometry

中文关键词:  液相色谱法  电感耦合等离子体质谱法  砷  形态化合物  大米

英文关键词:liquid chromatography  inductively coupled plasma mass spectrometry  arsenic  morphology compounds  rice

中文摘要:

      目的 建立液相色谱—电感耦合等离子体质谱法测定大米中砷形态化合物的分析方法。方法 样品经硝酸提取, 采用Dionex lonPac AS7色谱柱, 5 mmoL/L和10 mmoL/L碳酸铵溶液作为流动相进行梯度洗脱, 液相色谱分离, 电感耦合等离子体质谱进行定性和定量分析。结果 在1.0~100.0 μg/L浓度范围内各砷形态线性良好, 相关系数r均优于0.999, 方法加标回收率为84.5%~112.1%, 相对标准偏差<5%。结论 本研究建立的方法准确、可靠、灵敏度高, 适用于大米中砷形态化合物的定量检测。

英文摘要:

      Objective To establish a method for the determination of arsenic morphology compounds in rice by liquid chromatography-inductively coupled plasma mass spectrometry. Methods Samples were extracted with nitric acid, using Dionex lonPac AS7 chromatographic column, with 5 mmoL/L and 10 mmoL/L ammonium carbonate solutions as mobile phase for gradient elution, and the samples were separated by liquid chromatography, and inductively coupled plasma mass spectrometry was used for qualitative and quantitative analysis. Results Various arsenic forms had good linear relationships in the range of 1.0?100.0 g/L, and the correlation coefficient was higher than 0.999. The spiked recoveries were obtained from 84.5% to 112.1%, with the relative standard deviation was less than 5%. Conclusion This method is accurate, reliable and sensitive, which is suitable for the quantitative determination of arsenic morphological compounds in rice.